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Heritable gene targeting in zebrafish using customized TALENs

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Figure 1: Targeted and heritable disruption of the tnikb gene in zebrafish using TALENs.

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Acknowledgements

The authors thank R. Jiao, W. Wang and X. Tong for helpful discussions; H. Xin, C. Zhang and Z. Jiang for help in the luciferase SSA recombination assay; F. Tang, Y. Zhang and P. Xu at the Biodynamic Optical Imaging Center (BIOPIC) for help in deep sequencing; Y. Gao and J. Zhang for laboratory management and technical support; and Y. Jia, J. Chen and H. Cui for maintenance of zebrafish facility. This work was partially supported by grants from National Natural Science Foundation of China (NSFC) (30730056) and 973 program (2011CBA01000, 2007CB914502).

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Authors and Affiliations

  1. Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing, P.R. China

    Peng Huang, An Xiao, Zuoyan Zhu & Bo Zhang

  2. Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University, Shenzhen Graduate School, Shenzhen University Town, Shenzhen, P.R. China

    Peng Huang & Shuo Lin

  3. College of Plant Protection, Nanjing Agricultural University, Nanjing, P.R. China

    Mingguo Zhou

  4. Department of Molecular, Cell & Developmental Biology, University of California, Los Angeles, Los Angeles, California, USA

    Shuo Lin

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  1. Peng Huang
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  2. An Xiao
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  3. Mingguo Zhou
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  4. Zuoyan Zhu
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  5. Shuo Lin
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Correspondence to Shuo Lin or Bo Zhang.

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The authors declare no competing financial interests.

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Supplementary Text and Figures

Supplementary Figures 1–7, Supplementary Tables 1–3, Supplementary Sequences and Supplementary Methods (PDF 1245 kb)

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Huang, P., Xiao, A., Zhou, M. et al. Heritable gene targeting in zebrafish using customized TALENs. Nat Biotechnol 29, 699–700 (2011). https://doi.org/10.1038/nbt.1939

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