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Anti-galactose-α-1,3-galactose IgE from allergic patients does not bind α-galactosylated glycans on intact therapeutic antibody Fc domains

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Figure 1: Binding of IgE antibodies in serum samples of 20 meat allergy patients to therapeutic mAbs in different production systems and the interaction of cetuximab, infliximab or isolated fragments thereof with α-Gal–specific IgE measured in an IgE inhibition RIA.

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Acknowledgements

We thank M. Geukes, A.H. Simmelink and N.I.L. Derksen for valuable technical assistance, and R. Taylor for his enthusiastic support.

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Author notes

  1. Jeroen J Lammerts van Bueren and Theo Rispens: These authors contributed equally to this work.

Authors and Affiliations

  1. Genmab, Utrecht, The Netherlands

    Jeroen J Lammerts van Bueren, Sandra Verploegen, Patrick H C van Berkel, Jan G J van de Winkel & Paul W H I Parren

  2. Sanquin Research, Amsterdam, The Netherlands

    Theo Rispens, Tjitske van der Palen-Merkus & Steven Stapel

  3. Asthma and Allergic Diseases Center, University of Virginia, Charlottesville, Virginia, USA

    Lisa J Workman, Hayley James & Thomas A E Platts-Mills

  4. Department of Immunology, Immunotherapy Laboratory, University Medical Center Utrecht, Utrecht, The Netherlands

    Jan G J van de Winkel

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  1. Jeroen J Lammerts van Bueren
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Corresponding author

Correspondence to Paul W H I Parren.

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Competing interests

J.J.L.v.B., S.V., P.H.C.v.B, J.G.J.v.d.W. and P.W.H.I.P. are Genmab employees and Genmab warrants and/or stock. T.R. has received research support rhgouh his institution from Genmab.

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Supplementary Figures 1–5, Supplementary Tables 1 and 2, and Supplementary Methods (PDF 1046 kb)

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van Bueren, J., Rispens, T., Verploegen, S. et al. Anti-galactose-α-1,3-galactose IgE from allergic patients does not bind α-galactosylated glycans on intact therapeutic antibody Fc domains. Nat Biotechnol 29, 574–576 (2011). https://doi.org/10.1038/nbt.1912

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