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Human embryonic stem cells reveal recurrent genomic instability at 20q11.21

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Abstract

By analyzing five human embryonic stem (hES) cell lines over long-term culture, we identified a recurrent genomic instability in the human genome. An amplification of 2.5–4.6 Mb at 20q11.21, encompassing ∼23 genes in common, was detected in four cell lines of different origins. This amplification, which has been associated with oncogenic transformation, may provide a selective advantage to hES cells in culture.

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Figure 1: Genomic instability at 20q11.21 in SA01.

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Acknowledgements

This study has been supported in part by additional grants from Medicen Paris Region (IngeCELL), EC (FP6, STEM-HD) and ANR (HESCREEN). The authors thank K. Sermon (AZ-VUB, Brussels) for kindly providing VUB cell lines.

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Authors and Affiliations

  1. INSERM/UEVE UMR-861, I-STEM, AFM, Institute for Stem cell Therapy and Exploration of Monogenic diseases, 5 rue Henri Desbruères, Evry, 91030, cedex, France

    Nathalie Lefort, Maxime Feyeux, Marc Peschanski & Anselme L Perrier

  2. Service de biologie et génétique de la reproduction, INSERM U782, Hôpital Antoine Béclère, Université Paris 11, Clamart, France

    Cécile Bas & Gerard Tachdjian

  3. INSERM U602/Université Paris-Sud 11, Hôpital Paul Brousse, Villejuif, France

    Olivier Féraud & Annelise Bennaceur-Griscelli

Authors
  1. Nathalie Lefort
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  2. Maxime Feyeux
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  3. Cécile Bas
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  4. Olivier Féraud
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  5. Annelise Bennaceur-Griscelli
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  6. Gerard Tachdjian
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  7. Marc Peschanski
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  8. Anselme L Perrier
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Contributions

N.L., M.P. and A.L.P. designed the study. N.L. carried out and analyzed CGH and SNP experiments. C.B. carried out the FISH experiments. N.L. and M.F. did all hES cell culture, except for that of H1 cells, which were cultured by O.F. The FISH experiments were designed and analyzed by G.T., A.B.-G. and C.B. A.B.-G. and G.T. edited the paper. N.L., M.P. and A.L.P. contributed to writing the paper.

Corresponding author

Correspondence to Anselme L Perrier.

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Supplementary Figures 1,2, Table 1, Methods (PDF 1046 kb)

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Lefort, N., Feyeux, M., Bas, C. et al. Human embryonic stem cells reveal recurrent genomic instability at 20q11.21. Nat Biotechnol 26, 1364–1366 (2008). https://doi.org/10.1038/nbt.1509

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  • DOI: https://doi.org/10.1038/nbt.1509

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