Abstract
Immunoglobulin gene sequence analysis is widely utilized for prognostication in chronic lymphocytic leukemia (CLL) and the definition of standardized procedures has allowed reliable and reproducible results. Occasionally, a straightforward interpretation of the sequences is not possible because of the so-called ‘problematic sequences’ that do not fit the ‘classic’ interpretation and pose scientific questions at the cross-road between hematology and immunology. Thanks to a dedicated effort within the European Research Initiative on CLL (ERIC), we have now the possibility to present such cases, offer a scientific explanation and propose recommendations in terms of prognostication.
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Acknowledgements
We thank Professor Marie-Paule Lefranc and Dr Veronique Giudicelli, Laboratoire d’Immunogenetique Moleculaire, LIGM, Universite Montpellier II, Montpellier, France, for both their long-standing collaboration with our group and their invaluable guidance to immunoglobulin gene sequence analysis. We also thank the following members of our groups for their assistance with both technical and methodological issues as well as the interpretation of immunoglobulin gene sequence results: Elena Arvaniti, Myriam Boudjoghra, Lone Bredo Pedersen, Victoria Bryant, Nicola Cahill, Antonis Dagklis, Vassiliki Douka, Ellen van Gastel-Mol, Mattias Jansson, Tatjana Smilevska, Gerard Tobin, Eugenia Tsakou, Ulf Thunberg and Isla Wheatley. Finally, we acknowledge the collaboration of Gabriele Strache who set up the webpage of the ERIC Review Board and is taking care of its maintenance. This work was supported by the Swedish Cancer Society and the Swedish Medical Research Council; the Program grant in Molecular Clinical Oncology-5 per mille # 9965 and Investigator grant, Associazione Italiana per la Ricerca sul Cancro Milano, Italy; PRIN-MIUR, Rome, Italy, Progetti Integrati Oncologia (PIO)–Ministero della Salute, Rome, Italy; the CARIPLO-Foundation, Milano, Italy; the Tenovus UK, Tenovus Solentside and the Experimental Cancer Medicine Centre (ECMC) network; the French Ministry of Health; ENosAI project (code 09SYN-13-880), co-funded by the EU and the Hellenic General Secretariat for Research and Technology; and the Greek Ministry of Health.
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Langerak, A., Davi, F., Ghia, P. et al. Immunoglobulin sequence analysis and prognostication in CLL: guidelines from the ERIC review board for reliable interpretation of problematic cases. Leukemia 25, 979–984 (2011). https://doi.org/10.1038/leu.2011.49
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DOI: https://doi.org/10.1038/leu.2011.49
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