Abstract
Rodent bioassays often require fresh, uncontaminated urine from a large number of mice. The authors describe a quick and simple method to collect moderate to large amounts of urine from individual mice using disposable plastic trays.
References
Externest, D., Meckelein, B., Schmidt, M.A. & Frey, A. Correlations between antibody immune responses at different mucosal effector sites are controlled by antigen type and dosage. Infect. Immun. 68, 3830–3839 (2000).
Kurien, B.T. & Scofield, R.H. Mouse urine collection using clear plastic wrap. Lab. Anim. 33, 83–86 (1999).
Smith, C.R., Felton, J.S. & Taylor, R.T. Description of a disposable individual-mouse urine collection apparatus. Lab. Anim. Sci. 31, 80–82 (1981).
West, R.W., Stanley, J.W. & Newport, G.D. Single-mouse urine collection and pH monitoring system. Lab. Anim. Sci. 28, 343–345 (1978).
Khosho, F.K., Kaufmann, R.C. & Amankwah, K.S. A simple and efficient method for obtaining urine samples from rats. Lab. Anim. Sci. 35, 513–514 (1985).
Hayashi, S. & Sakaguchi, T. Capillary tube urinalysis for small animals. Lab. Anim. Sci. 25(6), 781–782 (1975).
Fenske, M. Application of a new, simple method for quantitative collection of 24-hour urines in small laboratory animals: determination of basal excretion of proteins, creatinine, urea, electrolytes, and of free steroids. Z.Versuchstierkd. 32, 65–70 (1989).
Perissin, L., Zorzet, S., Rapozzi, V. & Giraldi, T. A non-invasive simple method for measurement of urinary excretion of melatonin in undisturbed mice. J. Pineal Res. 15, 138–140 (1993).
Acknowledgements
We would like to thank A*STAR (R-178-000-010-303), Republic of Singapore for funding as well as Lim Lay Hong and Lim Wei Seng for reading the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chew, J., Chua, K. Collection of Mouse Urine for Bioassays. Lab Anim 32, 48–50 (2003). https://doi.org/10.1038/laban0803-48
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/laban0803-48
- Springer Nature America, Inc.
This article is cited by
-
Nicotinic receptors promote susceptibility to social stress in female mice linked with neuroadaptations within VTA dopamine neurons
Neuropsychopharmacology (2022)
-
Maternal Supplementation of Low Dose Fluoride Alleviates Adverse Perinatal Outcomes Following Exposure to Intrauterine Inflammation
Scientific Reports (2019)
-
Apolipoprotein A1 mimetic peptide ATI-5261 reverses arterial stiffness at late pregnancy and early postpartum in a COMT−/− mouse model of preeclampsia
Clinical Hypertension (2018)