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Effects of daily restraint with and without injections on skeletal properties in C57BL/6NHsd mice

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Figure 1: Effect of daily animal handling and injection on body mass (a), thymus mass/body mass ratio (b) and neutrophil/lymphocyte ratio (c).
Figure 2: Effect of daily animal handling and injection on trabecular bone volume (a) and cortical bone area (b).
Figure 3: Effect of daily animal handling and injection on ultimate load of the tibia and femur.

References

  1. Turner, P. V., Brabb, T., Pekow, C. & Vasbinder, M. A. J. Am. Assoc. Lab. Anim. Sci. 50, 600–613 (2011).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Balcombe, J. P., Barnard, N. D. & Sandusky, C. Contemp. Top. Lab. Anim. Sci. 43, 42–51 (2004).

    CAS  PubMed  Google Scholar 

  3. Robling, A., Castillo, A. & Turner, C. H. Annu. Rev. Biomed. Eng. 8, 455 (2006).

    Article  CAS  Google Scholar 

  4. Kramer, K. et al. Altern. Lab. Anim. 32 Suppl. 1A, 177–181 (2004).

    CAS  PubMed  Google Scholar 

  5. Hankenson, F. C. et al. J. Am. Assoc. Lab. Anim. Sci. 52, 277–285 (2013).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Horn, M. J., Hudson, S. V., Bostrom, L. A. & Cooper, D. M. J. Am. Assoc. Lab. Anim. Sci. 51, 781–788 (2012).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Trammell, R. A., Cox, L. & Toth, L. A. Comp. Med. 62, 172–178 (2012).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Augustsson, H., Lindberg, L. & Höglund, A. U. Lab. Anim. 36, 271–281 (2002).

    Article  CAS  Google Scholar 

  9. Trammell, R. A. & Toth, L. A. Comp. Med. 61, 492–498 (2011).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Bollinger, A. P and Everds, N. The Laboratory Mouse. (Hedrich, H.J., ed.) Academic Press, Cambridge, MA. (2004).

    Google Scholar 

  11. Davis, A. K., Maney, D. L. & Maerz, J. C. Funct. Ecol. 22, 760–772 (2008).

    Article  Google Scholar 

  12. Bouxsein, M. L. et al. J. Bone Miner. Res. 20, 1085–1092 (2005).

    Article  Google Scholar 

  13. Berman, A. G., Clauser, C. A., Wunderlin, C., Hammond, M. A. & Wallace, J. M. PLoS ONE 10, e0130504–16 (2015).

    Article  Google Scholar 

  14. O'Brien, C. A. et al. Endocrinology 145, 1835–1841 (2004).

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported in part by NIH grants AR62002 (M.R.A.) and AR067221 (J.M.W.). The microCT utilized in this experiment was purchased through a NIH S10 grant (OD 016208). The authors would like to thank Karen Boschen for performing the blinded behavioral assessment.

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Authors and Affiliations

  1. Laboratory Animal Resource Center, Indiana University School of Medicine, Indianapolis, IN

    Rachel A Larsen, Jessica L Peveler, Joshuah B Klutzke & Debra L Hickman

  2. Department of Cellular & Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN

    Debra L Hickman

  3. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN

    Mohammad W Aref, Drew M Brown & Matthew R Allen

  4. Department of Biomedical Engineering, Indiana University-Purdue University of Indianapolis, Indianapolis, IN

    Joseph M Wallace & Matthew R Allen

  5. Roudebush Veterans Administration Medical Center, Indianapolis, IN

    Matthew R Allen

Authors
  1. Rachel A Larsen
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  2. Jessica L Peveler
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  3. Joshuah B Klutzke
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  4. Debra L Hickman
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Correspondence to Matthew R Allen.

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The authors declare no competing financial interests.

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Supplementary Materials and Methods, Supplementary Tables 1–5 (PDF 167 kb)

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Larsen, R., Peveler, J., Klutzke, J. et al. Effects of daily restraint with and without injections on skeletal properties in C57BL/6NHsd mice. Lab Anim 46, 299–301 (2017). https://doi.org/10.1038/laban.1295

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