Abstract
The gut microcolony assay has been used to measure damage to intestinal crypts by single and split doses of 3 alkylating agents: mechlorethamine hydrochloride (HN2), bis-chloroethyl-nitrosourea (BCNU) and isopropyl methane sulphonate (IMS). The single-dose survival curves for whole crypts were distinguished by extrapolation numbers (3.0, 176 and 1.5 respectively) that were lower than most previously published values for assay by irradiation. Significant sparing of crypts occurred when doses of HN2 or BCNU, but not IMS, were given in 2 equal fractions separated by more than 2 h. Deduced D0 values for those cells from which crypts regenerate were 1.9 mg/kg HN2, 19 mg/kg BCNU and 487 mg/kg IMS.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Rights and permissions
About this article
Cite this article
Moore, J. Ablation of murine jejunal crypts by alkylating agents. Br J Cancer 39, 175–181 (1979). https://doi.org/10.1038/bjc.1979.28
Issue Date:
DOI: https://doi.org/10.1038/bjc.1979.28
- Springer Nature Limited
This article is cited by
-
Estimates of the number of clonogenic cells in crypts of murine small intestine
Virchows Archiv B Cell Pathology Including Molecular Pathology (1987)
-
Relationship between surviving clonogenic crypt fraction and animal lethality after cytosine arabinoside (Ara-C) exposure
Virchows Archiv B Cell Pathology Including Molecular Pathology (1984)
-
Microcrystalline and three-dimensional network structure of plasticized poly(vinyl chloride)
Colloid & Polymer Science (1982)
-
Effects of radiation on normal tissues: Hypothetical mechanisms and limitations of in situ assays of clonogenicity
Radiation and Environmental Biophysics (1981)