Abstract
Chalone prepared from primary BALB/c mouse embryo fibroblasts caused a 33% reduction in incorporation of tritiated thymidine in the cultures of the second in vitro passage of BALB/c embryo fibroblasts, whereas chalone prepared from thymus, skin and spleen was without effect. Pretreatment of BALB/c secondary fibroblasts with the polyanions dextran sulphate and heparin abrogated the chalone effect. The polycations DEAE-dextran and polybrene were without effect. The effect of incubation with the dextran sulphate polyanion was reversed when followed by incubation with DEAE-dextran polycation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Rights and permissions
About this article
Cite this article
Ebbesen, P., Olsson, L. Chalone-like effect abrogated by dextran sulphate and heparin polyanion pretreatment of target cells. Br J Cancer 31, 649–652 (1975). https://doi.org/10.1038/bjc.1975.111
Issue Date:
DOI: https://doi.org/10.1038/bjc.1975.111
- Springer Nature Limited
This article is cited by
-
Characterization of a G1 inhibitor from old JB-1 ascites tumor fluid
Virchows Archiv B Cell Pathology (1976)