When DNA-binding proteins are tethered to dam methylase from Escherichia coli, adenine methylation is directed to eukaryotic target sites in vivo. Hybridization of methylated DNA to microarrays allows binding sites to be displayed genome-wide, providing a versatile alternative to chromatin immunoprecipitation.
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References
van Steensel, B., Delrow, J. & Henikoff, S. Nature Genet. 27, 304–308 (2001).
Kirianov, G.I., Smirnova, T.A., Isaeva, L.V., Vaniushin, B.F. & Bur'ianov, I.I. Biokhimiia 46, 1887–1895 (1981).
Singh, J. & Klar, A.J. Genes Dev. 6, 186–196 (1992).
Wines, D.R., Talbert, P.B., Clark, D.V. & Henikoff, S. Chromosoma 104, 332–340 (1996).
van Steensel, B. & Henikoff, S. Nature Biotechnol. 18, 424–428 (2000).
Henikoff, S. Biochim. Biophys. Acta. 1470, 1–8 (1999).
The Cold Spring Harbor Laboratory, Washington University & PE Biosystems Arabidopsis Sequencing Consortium. Cell 100, 377–386 (2000).
Ren, B. et al. Science 290, 2306–2309 (2000).
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Martienssen, R. Chipping away at chromatin. Nat Genet 27, 240–241 (2001). https://doi.org/10.1038/85787
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DOI: https://doi.org/10.1038/85787
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