Abstract
Manipulation or non-physiological embryo culture environments can lead to defective fetal programming in livestock. Our demonstration of reduced fetal methylation and expression of ovine IGF2R suggests pre-implantation embryo procedures may be vulnerable to epigenetic alterations in imprinted genes. This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Young, L.E., Sinclair, K.D. & Wilmut, I. Rev. Reprod. 3, 155–163 (1998).
Young, L.E. & Fairburn, H.R. Theriogenology 53, 627–648 (2000).
Leighton, P.A., Ingram, R.S., Eggenschwiler, J., Efstratiadis, A. & Tilghman, S.M. Nature 375, 34–39 (1995).
Eggenschwiler, J. et al. Genes Dev. 11, 3128–3142 (1997).
Wang, Z.Q., Fung, M.R., Barlow, D.P. & Wagner, E.F. Nature 372, 464–467 (1994).
Lau, M.M.H. et al. Genes Dev. 8, 2953–2963 (1994).
Dean, W. et al. Development 125, 2273–2282 (1998).
Sinclair, K.D. et al. Theriogenology 49, 218 (1998).
Vogt, T. et al. Clin. Cancer Res. 4, 791–797 (1998).
Armstrong, D.G., McKay, C.O., Morrell, D.J. & Goddard, C. J. Endocrinol. 120, 373–378 (1989).
Li, E., Bestor, T.H. & Jaenisch, R. Cell 69, 915–926 (1992).
Gutierrez, C.G., Campbell, B.K., Armstrong, D.G. & Webb, R. J. Endocrinol. 153, 231–240 (1997).
Ludwig, T. et al. Dev. Biol. 177, 517–535 (1996).
Jones, J.I. & Clemmons, D.R. Endocr. Rev. 16, 3–34 (1995).
Butler, J.H. & Gluckman, P.D. J. Endocrinol. 109, 333–338 (1986).
Acknowledgements
We thank P. Lobel for the IGF2R antibody and purified IGF2R. This work was supported by MAFF and SOAEFD.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Young, L., Fernandes, K., McEvoy, T. et al. Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture. Nat Genet 27, 153–154 (2001). https://doi.org/10.1038/84769
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/84769
- Springer Nature America, Inc.
This article is cited by
-
Association of adverse birth outcomes with in vitro fertilization after controlling infertility factors based on a singleton live birth cohort
Scientific Reports (2022)
-
DNA methylation and gene expression changes in mouse pre- and post-implantation embryos generated by intracytoplasmic sperm injection with artificial oocyte activation
Reproductive Biology and Endocrinology (2021)
-
Comprehensive analysis of whole genome methylation in mouse blastocysts cultured with four different constituents following in vitro fertilization
Middle East Fertility Society Journal (2020)
-
The growth and development conditions in mouse offspring derived from ovarian tissue cryopreservation and orthotopic transplantation
Journal of Assisted Reproduction and Genetics (2020)
-
Cancer risk in children born after assisted reproductive technology
Gynäkologische Endokrinologie (2020)