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New wine from old barrels

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Phosphoribosylanthranilate isomerase (PRAI) and indole-3-glycerol phosphate synthase (IGPS) are distantly related α/β-barrel enzymes that catalyze successive steps in tryptophan biosynthesis. A ‘new’ PRAI was evolved from an ‘old’ IGPS by random mutagenesis followed by DNA shuffling and in vivo selection.

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Figure 1: Reactions catalyzed by phosphoribosylanthranilate isomerase (PRAI) and indole-3-glycerol phosphate synthase (IGPS).
Figure 2: The α/β-barrel folds of IGPS and PRAI.
Figure 3

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  1. Departments of Biochemistry and Chemistry, University of Illinois, 600 South Mathews Avenue, Urbana, 61801, Illinois, USA

    John A. Gerlt

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  1. John A. Gerlt
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Gerlt, J. New wine from old barrels. Nat Struct Mol Biol 7, 171–173 (2000). https://doi.org/10.1038/73249

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