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An economic method for the fluorescent labeling of PCR fragments

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Abstract

A poor man's approach to genotyping for research and high-throughput diagnostics.

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Figure 1: Amplification scheme for the one-tube, single-reaction nested PCR method.
Figure 2: (A) The informative pedigree of a family in which the MaoA locus on the X chromosome was haplotyped.

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Schuelke, M. An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18, 233–234 (2000). https://doi.org/10.1038/72708

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