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Genome editing

The domestication of Cas9

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The enzyme Cas9 is used in genome editing to cut selected DNA sequences, but it also creates breaks at off-target sites. Protein engineering has now been used to make Cas9 enzymes that have minimal off-target effects. See Article p.490

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Figure 1: Taming a wild enzyme.

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References

  1. Kleinstiver, B. P. et al. Nature 529, 490–495 (2016).

    Article  ADS  CAS  Google Scholar 

  2. Slaymaker, I. M. et al. Science 351, 84–88 (2016).

    Article  ADS  CAS  Google Scholar 

  3. Carroll, D. Annu. Rev. Biochem. 83, 409–439 (2014).

    Article  CAS  Google Scholar 

  4. Doudna, J. A. & Charpentier, E. Science 346, 1258096 (2014).

    Article  Google Scholar 

  5. Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S. & Gregory, P. D. Nature Rev. Genet. 11, 636–646 (2010).

    Article  CAS  Google Scholar 

  6. Bolukbasi, M. F., Gupta, A. & Wolfe, S. A. Nature Methods 13, 41–50 (2015).

    Article  Google Scholar 

  7. Tsai, S. Q. et al. Nature Biotechnol. 33, 187–197 (2015).

    Article  CAS  Google Scholar 

  8. Jiang, F. & Doudna, J. A. Curr. Opin. Struct. Biol. 30, 100–111 (2015).

    Article  CAS  Google Scholar 

  9. Pavletich, N. P. & Pabo, C. O. Science 252, 809–817 (1991).

    Article  ADS  CAS  Google Scholar 

  10. Shalem, O., Sanjana, N. E. & Zhang, F. Nature Rev. Genet. 16, 299–311 (2015).

    Article  CAS  Google Scholar 

  11. Tebas, P. et al. N. Engl. J. Med. 370, 901–910 (2014).

    Article  CAS  Google Scholar 

  12. Sharma, R. et al. Blood 126, 1777–1784 (2015).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Fyodor Urnov is at Sangamo BioSciences, Richmond, California 94804, USA.,

    Fyodor Urnov

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  1. Fyodor Urnov
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Correspondence to Fyodor Urnov.

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The author declares competing financial interests: F.U. is a full-time employee of Sangamo BioSciences, Inc.

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Urnov, F. The domestication of Cas9. Nature 529, 468–469 (2016). https://doi.org/10.1038/529468a

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