A bacterial enzyme that uses guide RNA molecules to target DNA for cleavage has been adopted as a programmable tool to site-specifically modify genomes of cells and organisms, from bacteria and human cells to whole zebrafish.
This is a preview of subscription content, log in via an institution to check access.
References
Ishino, Y., Shinagawa, H., Makino, K., Amemura, M. & Nakata, A. J. Bacteriol. 169, 5429–5433 (1987).
Cong, L. et al. Science 339, 819–823 (2013).
Mali, P. et al. Science 339, 823–826 (2013).
Cho, S. W., Kim, S., Kim, J. M. & Kim, J. S. Nature Biotechnol. 31, 230–232 http://dx.doi.org/10.1038/nbt.2507 (2013).10.1038/nbt.2507
Hwang, W. Y. et al. Nature Biotechnol. 31, 227–229 http://dx.doi.org/10.1038/nbt.2501 (2013).10.1038/nbt.2501
Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L. A. Nature Biotechnol. 31, 233–239 http://dx.doi.org/10.1038/nbt.2508 (2013).10.1038/nbt.2508
Jinek, M. et al. Science 337, 816–821 (2012).
Jansen, R., Embden, J. D., Gaastra, W. & Schouls, L. M. Mol. Microbiol. 43, 1565–1575 (2002).
Haft, D. H., Selengut, J., Mongodin, E. F. & Nelson, K. E. PLoS Comput. Biol. 1, e60 (2005).
Mojica, F. J., Diez-Villasenor, C., Garcia-Martinez, J. & Soria, E. J. Mol. Evol. 60, 174–182 (2005).
Pourcel, C., Salvignol, G. & Vergnaud, G. Microbiology 151, 653–663 (2005).
Makarova, K. S., Grishin, N. V., Shabalina, S. A., Wolf, Y. I. & Koonin, E. V. Biol. Direct 1, 7 (2006).
Barrangou, R. et al. Science 315, 1709–1712 (2007).
Brouns, S. J. et al. Science 321, 960–964 (2008).
Deltcheva, E. et al. Nature 471, 602–607 (2011).
Garneau, J. E. et al. Nature 468, 67–71 (2010).
Sapranauskas, R. et al. Nucleic Acids Res. 39, 9275–9282 (2011).
Carroll, D. Mol. Ther. 20, 1658–1660 (2012).
Jinek, M. et al. eLIFE http://dx.doi.org/10.7554/eLife.00471 (2013).
Qi, L. S. et al. Cell 152, 1173–1183 (2013).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Charpentier, E., Doudna, J. Rewriting a genome. Nature 495, 50–51 (2013). https://doi.org/10.1038/495050a
Published:
Issue Date:
DOI: https://doi.org/10.1038/495050a
- Springer Nature Limited
This article is cited by
-
CRISPR/Cas tool designs for multiplex genome editing and its applications in developing biotic and abiotic stress-resistant crop plants
Molecular Biology Reports (2022)
-
Half a century of the reverse transcriptase—happy birthday!
Genome Biology (2021)
-
1H, 13C, 15 N backbone resonance assignment of the recognition lobe subdomain 3 (Rec3) from Streptococcus pyogenes CRISPR-Cas9
Biomolecular NMR Assignments (2021)
-
Brain transcriptome profile after CRISPR-induced ghrelin mutations in zebrafish
Fish Physiology and Biochemistry (2020)
-
Virus–Host Coevolution with a Focus on Animal and Human DNA Viruses
Journal of Molecular Evolution (2020)