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Cell-cycle related regulation of poly(A) polymerase by phosphorylation

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Abstract

THE poly(A) tail found on almost all eukaryotic messenger RNAs1,2 is important in enhancing translation initiation and determining mRNA stability3,4. Control of poly (A)-tail synthesis thus has the potential to be a key regulatory step in gene expression and is indeed known to be important during early development in many organisms5. To study a possible basis for such regulation, we examined phosphorylation of poly(A) polymerase (PAP) by p34cdc2/cyclin B (maturation/mitosis-promoting factor, MPF). We show here that PAP can be phosphorylated in vivo and in vitro by MPF. Consistent with this, PAP becomes hyperphosphorylated both during meiotic maturation ofXenopus laevis oocytes and in HeLa cells arrested at M phase, times in the cell-cycle when MPF is known to be active6,7. We show further that hyperphosphorylation by MPF dramatically reduces the activity of purified PAP, and that PAP isolated from mitotic HeLa cells is similarly inhibited by hyperphosphorylation. This repression probably contributes to the well established reductions in poly (A) + RNA and/or protein synthesis known to occur in M-phase cells8–15.

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References

  1. Keller, W. Cell 81, 829–832 (1995).

    Article  CAS  Google Scholar 

  2. Manley, J. L. Curr. Opin. Genet. Dev. 5, 222–228 (1995).

    Article  CAS  Google Scholar 

  3. Beelman, C. A. & Parker, R. Cell 81, 179–183 (1995).

    Article  CAS  Google Scholar 

  4. Curtis, D., Lehmann, R. & Zamore, P. D. Cell 81, 171–178 (1995).

    Article  CAS  Google Scholar 

  5. Wickens, M. Semin. Dev. Biol. 3, 399–412 (1992).

    Google Scholar 

  6. Kobayashi, H. et al. Cold Spring Harb. Symp. Quant. Biol. 56, 437–447 (1991).

    Article  CAS  Google Scholar 

  7. Norbury, C. & Nurse, P. Annu. Rev. Biochem. 61, 441–470 (1992).

    Article  CAS  Google Scholar 

  8. Stewart, D., Shaeffer, J. & Humphrey, M. Science 161, 791–793 (1968).

    Article  ADS  Google Scholar 

  9. Fan, H. & Penman, S. J. Mol. Biol. 50, 665–670 (1970).

    Article  Google Scholar 

  10. Wilt, F. H. Cell 11, 673–681 (1977).

    Article  CAS  Google Scholar 

  11. Sagata, N., Shiokawa, K. & Yamana, K. Dev. Biol. 77, 431–448 (1980).

    Article  CAS  Google Scholar 

  12. Kanki, J. P. & Newport, J. W. Dev. Biol. 146, 191–213 (1991).

    Google Scholar 

  13. Dambrough, C. & Ford, F. J. Dev. Biol. 71, 323–340 (1979).

    Article  Google Scholar 

  14. Pawlowski, P. J. & Rodriguez, L. V. Dev. Biol. 40, 71–77 (1974).

    Article  CAS  Google Scholar 

  15. Slater, D. W. & Slater, I. Nature 240, 333–337 (1972).

    Article  ADS  CAS  Google Scholar 

  16. Raabe, T., Bollum, F. J. & Manley, J. L. Nature 353, 229–234 (1991).

    Article  ADS  CAS  Google Scholar 

  17. Elledge, S. J. & Harper, J. W. Curr. Opin. Cell Biol. 6, 847–852 (1994).

    Article  CAS  Google Scholar 

  18. Vesely, J. et al. Eur. J. Biochem. 224, 771–786 (1994).

    Article  CAS  Google Scholar 

  19. Zhao, W. & Manley, J. L. Mol. Cell. Biol. 16, 2378–2386 (1996).

    Article  CAS  Google Scholar 

  20. Wahle, E., Martin, G., Schlitz, E. & Keller, W. EMBO J. 10, 4251–4252 (1991).

    Article  CAS  Google Scholar 

  21. Thuresson, A. et al. Proc. Natl Acad. Sci. USA 91, 979–983 (1994).

    Article  ADS  CAS  Google Scholar 

  22. Ballantyne, S. et al. RNA 1, 64–78 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Bienroth, S., Wahle, E., Suter-Crazolera, C. & Keller, W. J. Biol. Chem. 266, 19768–19776 (1991).

    CAS  PubMed  Google Scholar 

  24. Murthy, K. G. K. & Manley, J. L. J. Biol. Chem. 267, 14804–14811 (1992).

    CAS  PubMed  Google Scholar 

  25. Gerhart, J., Wu, M. & Kirschner, M. J. Cell. Biol. 98, 1247–1255 (1984).

    Article  CAS  Google Scholar 

  26. Manley, J. L. et al. Proc. Natl Acad. Sci. USA 77, 3855–3859 (1980).

    Article  ADS  CAS  Google Scholar 

  27. Wang, Y. & Prives, C. Nature 376, 88–91 (1995).

    Article  ADS  CAS  Google Scholar 

  28. Prives, C. & Foukal, D. Meth. Cell Biol. 36, 185–210 (1991).

    Article  CAS  Google Scholar 

  29. Martin, G. & Keller, W. EMBO J. 15, 2593–2603 (1996).

    Article  CAS  Google Scholar 

  30. Raabe, T., Murthy, K. G. K. & Manley, J. L. Mol. Cell. Biol. 14, 2946–2957 (1994).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Science, Columbia University, New York, New York, 10027, USA

    Diana Foukal Colgan, K. G. K. Murthy, Carol Prives & James L Manley

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  1. Diana Foukal Colgan
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  2. K. G. K. Murthy
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  3. Carol Prives
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  4. James L Manley
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Colgan, D., Murthy, K., Prives, C. et al. Cell-cycle related regulation of poly(A) polymerase by phosphorylation. Nature 384, 282–285 (1996). https://doi.org/10.1038/384282a0

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