Abstract
DAUGHTER cells with distinct fates can arise through intrinsically asymmetrical divisions1. Before such divisions, factors crucial for determining cell fates become asymmetrically localized in the mother cell2,3. In Caenorhabditis elegans, PAR proteins are required for the early asymmetrical divisions that establish embryonic polarity4–8, and are asymmetrically localized in early blastomeres9,10, although the mechanism of their distribution is not known. Here we report the identification in C. elegans of a non-muscle myosin II heavy chain (designated NMY-2) by means of its interaction with the PAR-1 protein, a putative Ser/Thr protein kinase. Furthermore, injections of nmy-2 antisense RNA into ovaries of adult worms cause embryonic partitioning defects and lead to mislocalization of PAR proteins. We therefore conclude that NMY-2 is required for establishing cellular polarity in C. elegans embryos.
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References
Horvitz, H. R. & Herskowitz, I. Cell 68, 237–255 (1992).
Rhyu, M. S., Jan, L. Y. & Jan, Y. N. Cell 76, 477–491 (1994).
Spana, E. P. & Doe, C. Q. Development 121, 3187–3195 (1995).
Kemphues, K. J., Priess, J. R., Morton, D. G. & Cheng, N. Cell 52, 311–320 (1988).
Kirby, C., Kusch, M. & Kemphues, K. Devl Biol. 142, 203–215 (1990).
Morton, D. G., Roos, J. M. & Kemphues, K. J. Genetics 130, 771–790 (1992).
Levitan, D. J., Boyd, L., Mello, C. C., Kemphues, K. J. & Stinchcomb, D. T. Proc. natn. Acad. Sci. U.S.A. 91, 6108–6112 (1994).
Cheng, N. N., Kirby, C. M. & Kemphues, K. J. Genetics 139, 549–559 (1995).
Guo, S. & Kemphues, K. J. Cell 81, 611–620 (1995).
Etemad-Moghadam, B., Guo, S. & Kemphues, K. J. Cell 83, 743–752 (1995).
Blanar, M. A. & Rutter, W. J. Science 256, 1014–1018 (1992).
Young, P. E., Richman, A. M., Ketchum, A. S. & Kiehart, D. P. Genes Dev. 7, 29–41 (1993).
Lin, R., Thompson, S. & Priess, J. Cell 83, 599–609 (1995).
Spudich, J. A. Cell Regul. 1, 1–11 (1989).
Kiehart, D. P. Cell 60, 347–350 (1990).
Hill, D. P. & Strome, S. Devl Biol. 125, 75–84 (1988).
Hill, D. P. & Strome, S. Development 108, 159–172 (1990).
Drubin, D. G. & Nelson, W. J. Cell 84, 335–344 (1996).
Jansen, R., Dowzer, C., Michaelis, C., Galova, M. & Nasmyth, K. Cell 84, 687–697 (1996).
Bobola, N., Jansen, R., Shin, T. H. & Nasmyth, K. Cell 84, 699–709 (1996).
Simons, M. et al. Circulation Res. 69, 530–539 (1991).
Shohet, R. V. et al. Proc. natn. Acad. Sci. U.S.A. 86, 7726–7730 (1989).
Ketchum, A. S., Stewart, C. T., Stewart, M. & Kiehart, D. P. Proc. natn. Acad. Sci. U.S.A. 87, 6316–6320 (1990).
Wilson, R. et al. Nature 368, 32–38 (1994).
Okkema, P. G. & Fire, A. Development 120, 2175–2186 (1994).
Sulston, J., Schierenberg, E., White, J. & Thomson, N. Devl Biol. 100, 67–119 (1983).
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Guo, S., Kemphues, K. A non-muscle myosin required for embryonic polarity in Caenorhabditis elegans. Nature 382, 455–458 (1996). https://doi.org/10.1038/382455a0
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DOI: https://doi.org/10.1038/382455a0
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