Skip to main content
SpringerLink
Log in

Regulation of receptor-mediated endocytosis by Rho and Rac

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

PINOCYTOSIS and membrane ruffling are among the earliest and most dramatic cellular responses to stimulation by growth factors or other mitogens1. The small Ras-related G proteins Rho and Rac have a regulatory role in membrane ruffling1–3 and activated Rho has been shown to stimulate pinocytosis when microinjected into Xenopus oocytes4. In contrast to these well established effects of Rho and Rac on plasma membrane morphology and bulk pinocytosis, there has been no evidence for their involvement in the regulation of receptor-mediated endocytosis in clathrin-coated pits. Here we show that activated Rho and Rac inhibit transferrin-receptor-mediated endocytosis when expressed in intact cells. Furthermore, we have reconstituted these effects in a cell-free system and established that Rho and Rac can regulate clathrin-coated vesicle formation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ridley, A. J. & Hall, A. Cell 70, 401–410 (1992).

    Article  CAS  Google Scholar 

  2. Hall, A. A. Rev. Cell Biol. 10, 31–54 (1994).

    Article  CAS  Google Scholar 

  3. Takai, Y., Sasaki, T., Tanake, K. & Nakanishi, H. Trends, biochem. Sci. 20, 227–231 (1995).

    Article  CAS  Google Scholar 

  4. Schmalzing, G. et al. J. Cell Biol. 130, 1319–1332 (1995).

    Article  CAS  Google Scholar 

  5. Smythe, E., Redelmeier, T. E. & Schmid, S. L. Meth. Enzym. 219, 223–234 (1992).

    Article  CAS  Google Scholar 

  6. Schmid, S. L. Trends Cell Biol. 3, 145–148 (1993).

    Article  CAS  Google Scholar 

  7. Bokoch, G. M., Boh., B. P. & Chuang, T.-H. J. biol. Chem. 269, 31674–31679 (1994).

    CAS  PubMed  Google Scholar 

  8. Ueda, T., Kikuchi, A., Ohgo, N., Yamamoto, J. & Takai, Y. J. biol. Chem. 265, 9373–9380 (1990).

    CAS  PubMed  Google Scholar 

  9. Lelias, J. M. et al. Proc. natn. Acad. Sci. U.S.A. 90, 1479–1483 (1993).

    Article  ADS  CAS  Google Scholar 

  10. Scherle, P., Behrens, T. & Staudt, L. M. Proc. natn. Acad. Sci. U.S.A. 90, 7568–7572 (1989).

    Article  ADS  Google Scholar 

  11. Aktories, K. & Hall, A. Trends pharmac. Sci. 10, 415–418 (1989).

    Article  CAS  Google Scholar 

  12. Nobes, C. D. & Hall, A. Cell 81, 53–62 (1995).

    Article  CAS  Google Scholar 

  13. Malcolm, K. C., Ross, A. H., Qui, R-G., Symons, M. & Exton, J. H. J. biol. Chem. 269, 25951–25954 (1994).

    CAS  PubMed  Google Scholar 

  14. Singer, W. D., Brown, H. A., Bokoch, G. M. & Sternweis, P. C. J. biol. Chem. 270, 14944–14950 (1995).

    Article  CAS  Google Scholar 

  15. Chong, L. D., Traynor-Kaplan, A., Bokoch, G. M. & Schwartz, M. A. Cell 79, 507–513 (1994).

    Article  CAS  Google Scholar 

  16. Hartwig, J. H. et al. Cell 82, 643–653 (1995).

    Article  CAS  Google Scholar 

  17. Tolias, K. F., Cantley, L. C. & Carpenter, C. L. J. biol. Chem. 270, 17656–17659 (1995).

    Article  CAS  Google Scholar 

  18. Bokoch, G. M., Vlattos, C. J., Wang, Y., Knaus, V. G. & Traynor-Kaplan, A. E. Biochem. J. 315, 775–779 (1996).

    Article  CAS  Google Scholar 

  19. Kotani, K. et al. EMBO J. 13, 2313–2321 (1994).

    Article  CAS  Google Scholar 

  20. Wennström, S. et al. Curr. Biol. 4, 385–393 (1994).

    Article  Google Scholar 

  21. Claque, M. J., Thorpe, C. & Jones, A. T. FEBS Lett. 367, 272–274 (1995).

    Article  Google Scholar 

  22. Lamaze, C. & Schmid, S. L. J. Cell Biol. 129, 47–54 (1995).

    Article  CAS  Google Scholar 

  23. Liscovitch, M. & Cantley, L. C. Cell 81, 659–662 (1995).

    Article  CAS  Google Scholar 

  24. DeCamilli, P., Emr, S. D., McPherson, P. S. & Novick, P. Science 271, 1533–1539 (1996).

    Article  ADS  CAS  Google Scholar 

  25. Seaman, M. N. J., Ball, C. L. & Robinson, M. S. J. Cell Biol. 123, 1093–1105 (1993).

    Article  CAS  Google Scholar 

  26. Lamaze, C. & Schmid, S. L. Curr. Opin. Cell Biol. 7, 573–580 (1995).

    Article  CAS  Google Scholar 

  27. Zhang, S. et al. J. biol. Chem. 270, 23934–23936 (1995).

    Article  CAS  Google Scholar 

  28. Damke, H., Baba, T., van der Bliek, A. M. & Schmid, S. L. J. Cell Biol 131, 69–80 (1995).

    Article  CAS  Google Scholar 

  29. Xu, X., Barry, D. C., Settleman, J., Schwartz, M. A. & Bokoch, G. M. J. biol. Chem. 269, 23569–23574 (1994).

    CAS  PubMed  Google Scholar 

  30. Chuang, T.-H., Xu, X., Knaus, U. G., Hart, M. J. & Bokoch, G. M. J. biol. Chem. 268, 775–778 (1993).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology, The Scripps Research Institute, 10666 N. Torrey Pines Road, La Jolla, California, 92037, USA

    Christophe Lamaze, Laura J. Terlecky, Gary M. Bokoch & Sandra L. Schmid

  2. Department of Immunology, The Scripps Research Institute, 10666 N. Torrey Pines Road, La Jolla, California, 92037, USA

    Tsung-Hsein Chuang & Gary M. Bokoch

Authors
  1. Christophe Lamaze
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tsung-Hsein Chuang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Laura J. Terlecky
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gary M. Bokoch
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sandra L. Schmid
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lamaze, C., Chuang, TH., Terlecky, L. et al. Regulation of receptor-mediated endocytosis by Rho and Rac. Nature 382, 177–179 (1996). https://doi.org/10.1038/382177a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/382177a0

  • Springer Nature Limited

This article is cited by

Search

Navigation