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Impaired migration but not differentiation of haematopoietic stem cells in the absence of β1 integrins

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Abstract

ADHESIVE interactions mediated by integrins of the β1 subfamily1 are thought to be critical in controlling differentiation and migration of blood cell precursors2–7. Here we report that chimaeric mice generated with β1-integrin-deficient embryonic stem (ES) cells8,9 lack β−/− cells in blood and in haematopoietic organs such as spleen, thymus and bone marrow. Chimaeric embryos contain β1,-null haematopoietic cells in the yolk sac and in fetal blood but not in fetal liver. We show that such β−/− haematopoietic stem cells derived from yolk sac of 10.5-day-old chimaeric embryos readily generate erythroid and myeloid colonies and that β1−/− ES cells can differentiate into mature B lymphocytes in vitro. Our results indicate that haematopoietic stem cells lacking β1, integrins can form and differentiate into different lineages but cannot colonize the fetal liver.

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References

  1. Hynes, R. O. Cell 69, 11–25 (1992).

    Article  CAS  Google Scholar 

  2. Patel, V. P. & Lodish, H. F. J. Cell Biol. 102, 449–456 (1986).

    Article  CAS  Google Scholar 

  3. Patel, V. P. & Lodish, H. F. J. Cell Biol. 105, 3105–3118 (1987).

    Article  CAS  Google Scholar 

  4. Giancotti, F., Comoglio, P. & Tarone, G. J. Cell Biol. 103, 429–437 (1986).

    Article  CAS  Google Scholar 

  5. Papayannopoulou, T. & Brice, M. Blood 79, 1686–1694 (1992).

    CAS  PubMed  Google Scholar 

  6. Williams, D., Rios, M., Stephens, C. & Patel, V. P. Nature 352, 438–441 (1992).

    Article  ADS  Google Scholar 

  7. Ruiz, P., Wiles, M. V. & Imhof, B. Eur. J. Immun. 25, 2034–2041 (1995).

    Article  CAS  Google Scholar 

  8. Fässler, R. et al. J. Cell Biol. 128, 979–988 (1995).

    Article  Google Scholar 

  9. Fässler, R. & Meyer, M. Genes Dev. 9, 1896–1908 (1995).

    Article  Google Scholar 

  10. Pardanaud, L., Yassine, F. & Dieterlen-Lievre, F. Development 105, 473–485 (1989).

    CAS  PubMed  Google Scholar 

  11. Wilt, F. H. Science 147, 1588–1590 (1965).

    Article  ADS  CAS  Google Scholar 

  12. Johnson, G. R. & Moore, M. A. Nature 258, 726–728 (1975).

    Article  ADS  CAS  Google Scholar 

  13. Chen, J., Lansford, R., Stewart, V., Young, F. & Alt, F. W. Proc. natn. Acad. Sci. U.S.A. 90, 4528–4532 (1993).

    Article  ADS  CAS  Google Scholar 

  14. Potocnik, A. J., Nielsen, P. J. & Eichmann, K. EMBO J. 13, 5274–5283 (1994).

    Article  CAS  Google Scholar 

  15. Yang, J. T., Rayburn, H. & Hynes, R. O. Development 121, 549–560 (1995).

    CAS  PubMed  Google Scholar 

  16. Tedder, T. F., Steeber, D. A. & Pizcueta, P. J. exp. Med. 181, 2259–2264 (1995).

    Article  CAS  Google Scholar 

  17. Subramaniam, M. et al. J. exp. Med. 181, 2277–2282 (1995).

    Article  CAS  Google Scholar 

  18. Labow, M. A. et al. Immunity 1, 709–720 (1994).

    Article  CAS  Google Scholar 

  19. Anderson, D. C. & Springer, T. A. A. Rev. Med. 38, 175–194 (1987).

    Article  CAS  Google Scholar 

  20. Springer, T. A. Cell 76, 301–314 (1994).

    Article  CAS  Google Scholar 

  21. Moore, K. A. & Belmont, J. W. in Gene Targeting (ed. Joyner, A.) 63–106 (Oxford University Press, Oxford, UK, 1993).

    Google Scholar 

  22. Pevny, L. et al. Development 121, 163–172 (1995).

    CAS  PubMed  Google Scholar 

  23. Leptin, M. et al. Eur. J. Immun. 14, 534–542 (1984).

    Article  CAS  Google Scholar 

  24. Iglesias, A., Hansen-Hagge, T., Von Bonin, A. & Weltzien, H. U. Eur. J. Immun. 22, 335–341 (1992).

    Article  CAS  Google Scholar 

  25. Bottger, B. A., Hedin, U., Johansson, S. & Thyberg, J. Differentiation 41, 158–167 (1989).

    Article  CAS  Google Scholar 

  26. Schlissel, M. S. & Baltimore, D. Cell 58, 1001–1007 (1989).

    Article  CAS  Google Scholar 

  27. Schlissel, M. S. et al. J. exp. Med. 173, 711–720 (1991).

    Article  CAS  Google Scholar 

  28. Cumano, A. & Paige, C. J. EMBO J. 11, 593–601 (1992).

    Article  CAS  Google Scholar 

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Author notes

  1. Reinhard Fässler: To whom correspondence should be addressed.

Authors and Affiliations

  1. Max Planck Institute for Biochemistry, 82152, Martinsried, Germany

    Emilio Hirsch, Ursula Hartmann & Reinhard Fässler

  2. Max Planck Institute for Psychiatry, 82152, Martinsried, Germany

    Antonio Iglesias

  3. Max Planck Institute for Immunobiology, Freiburg, 79108, Germany

    Alexandra J. Potocnik

Authors
  1. Emilio Hirsch
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  2. Antonio Iglesias
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  3. Alexandra J. Potocnik
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  4. Ursula Hartmann
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  5. Reinhard Fässler
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Hirsch, E., Iglesias, A., Potocnik, A. et al. Impaired migration but not differentiation of haematopoietic stem cells in the absence of β1 integrins. Nature 380, 171–175 (1996). https://doi.org/10.1038/380171a0

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  • DOI: https://doi.org/10.1038/380171a0

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