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A mammalian SRB protein associated with an RNA polymerase II holoenzyme

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Abstract

A LARGE multisubunit complex containing RNA polymerase II, general transcription factors and SRB regulatory proteins initiates transcription of class II genes in yeast cells1–4. The SRB proteins are a hallmark of this RNA polymerase II holoenzyme as they are found only in this complex, where they contribute to the response to regulators4–8. We have now isolated a human honiologue of the yeast SRB7 gene and used antibodies against human SRB7 protein to purify and characterize a mammalian RNA polymerase II holoenzyme containing the general transcription factors TFIIE and TFIIH. This holoenzyme is more responsive to transcriptional activators than core RNA polymerase II when assayed in the presence of coactivators.

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References

  1. Koleske, A. J. & Young, R. A. Nature 368, 466–469 (1994).

    Article  ADS  CAS  Google Scholar 

  2. Kim, Y. J., Bjorklund, S., Li, Y., Sayre, M. H. & Kornberg, R. E. Cell 77, 599–608 (1994).

    Article  CAS  Google Scholar 

  3. Thompson, C. M. & Young, R. A. Proc. natn. Acad. Sci. U.S.A. 92, 4587–4590 (1995).

    Article  ADS  CAS  Google Scholar 

  4. Koleske, A. J. & Young, R. A. Trends biochem. Sci. 20, 113–116 (1995).

    Article  CAS  Google Scholar 

  5. Koleske, A. J., Buratowski, S., Nonet, M. & Young, R. A. Cell 69, 883–894 (1992).

    Article  CAS  Google Scholar 

  6. Thompson, C. M., Koleske, A. J., Chao, D. M. & Young, R. A. Cell 73, 1361–1375 (1993).

    Article  CAS  Google Scholar 

  7. Liao, S. M. et al. Nature 374, 193–196 (1995).

    Article  ADS  CAS  Google Scholar 

  8. Hengartner, C. J. et al. Genes Dev. 9, 897–910 (1995).

    Article  CAS  Google Scholar 

  9. Shykind, B. M., Kim, J. & Sharp, P. A. Genes Dev. 9, 1354–1365 (1995).

    Article  CAS  Google Scholar 

  10. Stelzer, G., Goppelt, A., Lottspeich, F. & Meisterernst, M. Molec. cell. Biol. 14, 4712–4721 (1994).

    Article  CAS  Google Scholar 

  11. Ge, H. & Roeder, R. G. Cell 78, 513–523 (1994).

    Article  CAS  Google Scholar 

  12. Kretzschmar, M., Kaiser, K., Lottspeich, F. & Meisterernst, M. Cell 78, 525–534 (1994).

    Article  CAS  Google Scholar 

  13. Goodrich, J. A. & Tijan, R. Curr. Opin. Cell Biol. 6, 403–409 (1994).

    Article  CAS  Google Scholar 

  14. Merino, A., Madden, K. R., Lane, W. S., Champoux, J. J. & Reinberg, D. Nature 365, 227–232 (1993).

    Article  ADS  CAS  Google Scholar 

  15. Inostroza, J. A., Mermelstein, F. H., Ha, I., Lane, W. S. & Reinberg, D. Cell 70, 477–489 (1992).

    Article  CAS  Google Scholar 

  16. Kim, T. K., Zhao, Y., Ge, H., Bernstein, R. & Roeder, R. G. J. biol. Chem. 270, 10976–10981 (1995).

    Article  CAS  Google Scholar 

  17. Kretzschmar, M., Stelzer, G., Roeder, R. G. & Meisterernst, M. Molec. cell. Biol. 14, 3927–3937 (1994).

    Article  CAS  Google Scholar 

  18. Koleske, A. J., Chao, D. M. & Young, R. A. Meth. Enzym. (in the press).

  19. Ossipow, V., Tassan, J. P., Nigg, E. A. & Schibler, U. Cell 83, 137–146 (1995).

    Article  CAS  Google Scholar 

  20. Bassett, D. E. J. et al. Trends Genet. 11, 372–373 (1995).

    Article  CAS  Google Scholar 

  21. Boeke, J. D., Trueheart, J., Natsoulis, G. & Fink, G. R. Meth. Enzym. 154, 164–175 (1987).

    Article  CAS  Google Scholar 

  22. Thompson, N. E., Aronson, D. B. & Burgess, R. R. J. biol. chem. 265, 7069–7077 (1990).

    CAS  PubMed  Google Scholar 

  23. Mäkelä, T. P. et al. Proc. natn. Acad. Sci. U.S.A. 92, 5174–5178 (1995).

    Article  ADS  Google Scholar 

  24. Hodo, H. & Blatti, S. P. Biochemistry 16, 2334–2343 (1977).

    Article  CAS  Google Scholar 

  25. Drapkin, R. & Reinberg, D. Trends biochem. Sci. 19, 504–508 (1994).

    Article  CAS  Google Scholar 

  26. Ohkuma, Y., Sumimoto, H., Horikoshi, M. & Roeder, R. G. Proc. natn. Acad. Sci. U.S.A. 87, 9163–9167 (1990).

    Article  ADS  CAS  Google Scholar 

  27. Parvin, J. D., Shykind, B. M., Meyers, R. E., Kim, J. & Sharp, P. A. J. biol. Chem. 369, 18414–18421 (1994).

    Google Scholar 

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Authors and Affiliations

  1. Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts, 02142

    David M. Chao, Ellen L. Gadbois, Peter J. Murray, Michelle S. Sonu & Richard A. Young

  2. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    David M. Chao, Ellen L. Gadbois, Peter J. Murray, Michelle S. Sonu & Richard A. Young

  3. Division of Molecular Oncology and Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, 02115, USA

    Stephen F. Anderson & Jeffrey D. Parvin

Authors
  1. David M. Chao
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  2. Ellen L. Gadbois
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  4. Stephen F. Anderson
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  5. Michelle S. Sonu
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  6. Jeffrey D. Parvin
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  7. Richard A. Young
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Chao, D., Gadbois, E., Murray, P. et al. A mammalian SRB protein associated with an RNA polymerase II holoenzyme. Nature 380, 82–85 (1996). https://doi.org/10.1038/380082a0

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