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Sensitization of cells and retroviruses to human serum by (αl-3) galactosyltransferase

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Abstract

MAMMALIAN C-type retroviruses are inactivated by human serum1,2, following triggering of the classical complement cascade3. This may have inhibited transmission to humans of C-type oncoviruses from other mammals1. Indeed, the retro-viruses human immunodeficiency virus and human T-cell leukaemia virus are resistant to human complement4,5. Antibody-independent activation of human Clq, the first component of the classical pathway, by retroviral envelope proteins has been described6. However, retroviruses produced from human cells are resistant to inactivation by human complement7,8 and human serum is known to contain antibodies directed against carbohydrates on retroviral envelopes9–11. Gal(αl–3)Gal terminal carbohydrates are expressed by most mammals but are absent in humans, which lack a functional (αl–3)galactosyltransferase gene12,13.Here, we demonstrate that anti-Gal(αl–3)Gal antibodies in human serum inactivate retroviruses produced from animal cells. Expression of porcine (αl–3)galactosyltransferase14–16in human cells renders the cells and the retroviruses they produce sensitive to human serum.

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References

  1. Welsh, R. M., Cooper, N. R., Jensen, F. C. & Oldstone, M. B. A. Nature 257, 612–614 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Welsh, R. M., Jensen, F. C., Cooper, N. R. & Oldstone, M. B. A. Virology 74, 432–440 (1976).

    Article  PubMed  Google Scholar 

  3. Cooper, N. R., Jensen, F. C., Welsh, R. M. & Oldstone, M. B. A. J. exp. Med. 144, 970–984 (1976).

    Article  CAS  PubMed  Google Scholar 

  4. Banapour, B., Sernatinger, J. & Levy, J. A. Virology 152, 268–271 (1986).

    Article  CAS  PubMed  Google Scholar 

  5. Hoshino, H., Tanaka, H., Miwa, M. & Okada, H. Nature 310, 324–325 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  6. Bartholomew, R. M., Esser, A. F. & Muller-Eberhard, H. J. J. exp. Med. 147, 844–853 (1978).

    Article  CAS  PubMed  Google Scholar 

  7. Takeuchi, Y. et al. J. Virol. 68, 8001–8007 (1994).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Cosset, F. L., Takeuchi, Y., Battini, J. L., Weiss, R. A. & Collins, M. K. L. J. Virol. (in the press).

  9. Barbacid, M., Bolognesi, D. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 77, 1617–1621 (1979).

    Article  ADS  Google Scholar 

  10. Snyder, H. W. Jr & Fleissner, E. Proc. natn. Acad. Sci. U.S.A. 77, 1622–1626 (1980).

    Article  ADS  CAS  Google Scholar 

  11. Löwer, J., et al. Virology 109, 409–417 (1981).

    Article  PubMed  Google Scholar 

  12. Larsen, R. D. Rivera-Marrero, C. A., Ernst, L. K., Cummings, R. D. & Lowe, J. B. J. biol. Chem. 265, 7055–7061 (1990).

    CAS  PubMed  Google Scholar 

  13. Galili, U. & Swanson, K. Proc. natn. Acad. Sci. U.S.A. 88, 7401–7404 (1991).

    Article  ADS  CAS  Google Scholar 

  14. Gustafsson, K., Strahan, K. & Preece, A. Immun. Rev. 141, 59–70 (1994).

    Article  CAS  PubMed  Google Scholar 

  15. Strahan, K. M. et al. Immunogenetics 41, 101–105 (1995).

    Article  CAS  PubMed  Google Scholar 

  16. Sandrin, M. S. & McKenzie, I. F. Immun. Rev. 141, 169–190 (1994).

    Article  CAS  PubMed  Google Scholar 

  17. Hamadeh, R. M. et al. J. clin. Invest. 89, 1223–1235 (1992).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Galili, U., Mandrell, R. E., Hamadeh, R. M., Shohet, S. B. & Griffiss, J. M. Infect. Immun. 56, 1730–1737 (1988).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Galili, U., Anaraki, F., Thall, A., Hill-Black, C. & Radic, M. Blood 82, 2485–2493 (1993).

    CAS  PubMed  Google Scholar 

  20. Almeida, I. C., Krautz, G. M., Krettli, A. U. & Travassos, L. R. J. clin. Lab. Anal. 7, 307–316 (1993).

    Article  CAS  PubMed  Google Scholar 

  21. Oriol, R., Ye, Y., Koren, E. & Cooper, D. K. Transplantation 56, 1433–1442 (1993).

    Article  CAS  PubMed  Google Scholar 

  22. Sandrin, M. S., Vaughan, H. A., Dabkowski, P. L. & McKenzie, I. F. Proc. natn. Acad. Sci. U.S.A. 90, 11391–11395 (1993).

    Article  ADS  CAS  Google Scholar 

  23. Repik, P. M., Strizki, J. M. & Galili, U. J. gen. Virol. 75, 1177–1181 (1994).

    Article  CAS  PubMed  Google Scholar 

  24. Welsh, R. M. J. Immun. 118, 348–354 (1977).

    CAS  PubMed  Google Scholar 

  25. Hirsch, R. L., Griffin, D. E. & Winkelstein, J. A. J. Immun. 127, 1740–1743 (1981).

    CAS  PubMed  Google Scholar 

  26. Fabre, J. W. Nature Med. 1, 403–404 (1995).

    Article  CAS  PubMed  Google Scholar 

  27. McCurry, K. R. et al. Nature Med. 1, 423–427 (1995).

    Article  CAS  PubMed  Google Scholar 

  28. Cosset, F. L. et al. J. Virol. 69, 6314–6322 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  29. McKenzie, I. F. C. et al. Lancet ii, 386–387 (1968).

    Article  Google Scholar 

  30. Battini, J. L., Heard, J. M. & Danos, O. J. Virol. 66, 1468–1475 (1992).

    CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK

    Yasuhiro Takeuchi, Colin D. Porter, Francois-Loic Cosset, Robin A. Weiss  & Mary K. L. Collins

  2. Institute of Child Health, University of London, 30 Guilford Street, London, WC1N 1EH, UK

    Karen M. Strahan, Andrew F. Preece & Kenth Gustafsson

Authors
  1. Yasuhiro Takeuchi
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  2. Colin D. Porter
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  3. Karen M. Strahan
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  4. Andrew F. Preece
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  5. Kenth Gustafsson
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  6. Francois-Loic Cosset
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  7. Robin A. Weiss
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  8. Mary K. L. Collins
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Takeuchi, Y., Porter, C., Strahan, K. et al. Sensitization of cells and retroviruses to human serum by (αl-3) galactosyltransferase. Nature 379, 85–88 (1996). https://doi.org/10.1038/379085a0

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