Abstract
WITHIN the Hoxb homeobox gene complex, Hoxb-1 is the earliest member expressed in the mesoderm and neuroectoderm of primitive streak and presomite embryos, preceding rhombomere-restricted expression in the hind brain1–7. Ectopic exposure of embryos to retinoic acid alters spatial aspects of Hox gene expression patterns8–15. However, the role of retinoids in regulating these genes during normal development is unclear. We have now identified two enhancers, 3′ of the mouse Hoxb-1 gene, which together reconstruct the early endogenous expression pattern and mediate the early ectopic response to retinoic acid. Furthermore, these regions are functionally conserved in both chicken and pufferfish (Fugu rubripes)16 Hoxb-1 genes. The enhancer that controls the retinoic acid response, and regulates expression predominantly in neuroectoderm, contains a retinoic acid response element (RARE). Point mutations in the RARE abolish expression in neuroectoderm. Therefore, this RARE is not only involved in the ectopic response to retinoic acid, but is also essential for establishing aspects of the early Hoxb-l expression pattern.
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References
Wilkinson, D. et al. Nature 341, 405–409 (1989).
Murphy, P., Davidson, D. & Hill, R. Nature 341, 156–159 (1989).
Frohman, M., Boyle, M. & Martin, G. Development 110, 589–607 (1990).
Sundin, O. & Eichele, G. Genes Dev. 4, 1267–1276 (1990).
Maden, M. et al. Development 111, 35–44 (1991).
Guthrie, S. et al. Nature 356, 157–159 (1992).
Murphy, P. & Hill, R. Development 111, 61–74 (1991).
Conlon, R. & Rossant, J. Development 116, 357–368 (1992).
Marshall, H. et al. Nature 360, 737–741 (1992).
Sundin, O. & Eichele, G. Development 114, 841–852 (1992).
Kessel, M. Neuron 10, 379–393 (1993).
Morriss-Kay, G. et al. EMBO J. 10, 2985–2996 (1991).
Kessel, M. & Gruss, P. Cell 67, 89–104 (1991).
Dolle, P. et al. Nature 342, 767–772 (1989).
Izpisua-Belmonte, J.-C. et al. Nature 350, 585–589 (1991).
Brenner, S. et al. Nature 366, 265–268 (1993).
Leid, M., Kastner, P. & Chambon, P. Trends biochem. Sci. 17, 427–433 (1992).
Stunnenberg, H. Bioessays 15, 309–315 (1993).
Kliewer, S. A. et al. Nature 355, 446–449 (1992).
Durston, A. et al. Nature 340, 140–144 (1989).
Ruiz i Altaba, A. & Jessell, T. Genes Dev. 5, 175–187 (1991).
Ruiz i Altaba, A. & Jessell, T. Development 112, 945–958 (1991).
Papalopulu, N. et al. Development 113, 1145–1159 (1991).
Sive, H. & Cheng, P. Genes Dev. 5, 1321–1332 (1991).
Sive, H. et al. Genes Dev. 4, 932–942 (1990).
Holder, N. & Hill, J. Development 113, 1159–1170 (1991).
Langston, A. W. & Gudas, L. J. Mechanisms of Development 38, 217–228 (1992).
Whiting, J. et al. Genes Dev. 5, 2048–2059 (1991).
Yee, S.-P. & Rigby, P. W. R. Genes Dev. 7, 1277–1289 (1993).
Popperl, H. & Featherstone, M. S. Molec. cell. Biol. 13, 257–265 (1993).
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Marshall, H., Studer, M., Pöpperl, H. et al. A conserved retinoic acid response element required for early expression of the homeobox gene Hoxb-1. Nature 370, 567–571 (1994). https://doi.org/10.1038/370567a0
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DOI: https://doi.org/10.1038/370567a0
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