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Direct role for Myc in transcription initiation mediated by interactions with TFII-I

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Abstract

THE nuclear proto-oncoprotein Myc has been implicated in the control of cell proliferation and differentiation1–3. Myc participates in transcription4,5 and belongs to the basic–helix–loop–helix (bHLH) family of regulatory proteins2,3. Here we show that Myc interacts with TFII-I, a transcription initiation factor that activates core promoters through an initiator element (Inr)6. As previously observed for the bHLH activator USF6, Myc was found to interact cooperatively with TFII-I at both Inr and upstream E-box promoter elements. However, in this case Myc interactions with TFII-I at the Inr lead to an inhibition of transcription initiation. This inhibition is selective for a TFII-I-dependent (as opposed to TFIIA-dependent) initiation pathway and correlates with the prevention of complex formation between the TATA-binding protein TBP (TFIIDτ), TFII-I and the promoter. TBP probably interacts with Myc, but only slowly. These observations indicate that Myc has the potential to interact physically and functionally with components of the general transcription machinery.

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References

  1. Cole, M. D. A. Rev Genet. 20, 361–384 (1986).

    Article  CAS  Google Scholar 

  2. Luscher, B. & Eisenman, R. N. Genes Dev. 4, 2025–20435 (1990).

    Article  CAS  Google Scholar 

  3. Pendergast, G. C. & Ziff, E. B. Trends genet. Sci. 8, 91–96 (1992).

    Article  Google Scholar 

  4. Kretzner, L., Blackwood, E. M. & Eisenman, R. N. Nature 359, 426–429 (1992).

    Article  ADS  CAS  Google Scholar 

  5. Amati, B. et al. Nature 359, 423–426 (1992).

    Article  ADS  CAS  Google Scholar 

  6. Roy, A. L. Meisterernst, M., Pognonec, P. & Roeder, R. G. Nature 354, 245–248 (1991).

    Article  ADS  CAS  Google Scholar 

  7. Gregor, P. D., Sawadogo, M. & Roeder, R. G. Genes Dev. 4, 1730–1740 (1990).

    Article  CAS  Google Scholar 

  8. Pognonec, P. & Roeder, R. G. Molec. cell. Blol. 11, 5125–5136 (1991).

    Article  CAS  Google Scholar 

  9. Murre, C., Schonleber-McCaw, P. & Baltimore, D. Cell 56, 777–783 (1989).

    Article  CAS  Google Scholar 

  10. Murre, C. et al. Cell 58, 537–544 (1989).

    Article  CAS  Google Scholar 

  11. Davis, R. L., Cheng, P. F., Lassar, A. B. & Weintraub, H. Cell 60, 733–746 (1990).

    Article  CAS  Google Scholar 

  12. Jones, N. Cell 61, 9–11 (1990).

    Article  CAS  Google Scholar 

  13. Lamb, P. & McKnight, S. L. Trends biochem. Sci. 16, 417–422 (1991).

    Article  CAS  Google Scholar 

  14. Blackwell, T. K. et al. Science 250, 1149–1152 (1990).

    Article  ADS  CAS  Google Scholar 

  15. Blackwood, E. & Eisenman, R. N. Science 251, 1211–1217 (1991).

    Article  ADS  CAS  Google Scholar 

  16. Pendergast, G. C., Lawe, D. & Ziff, E. B. Cell 65, 395–407 (1991).

    Article  Google Scholar 

  17. Roeder, R. G. Trends biochem. Sci. 16, 402–408 (1991).

    Article  CAS  Google Scholar 

  18. Roy, A. L., Malik, S., Meisterernst, M. & Roeder, R. G. Nature 365, 355–359 (1993).

    Article  ADS  CAS  Google Scholar 

  19. Meisterernst, M., Roy, A. L., Lieu, H. L. & Roeder, R. G. Cell 66, 981–993 (1991).

    Article  CAS  Google Scholar 

  20. Sawadogo, M. & Roeder, R. G. Cell 43, 165–175 (1985).

    Article  CAS  Google Scholar 

  21. Blackwood, E. M., Luscher, B. & Eisenman, R. N. Genes Dev. 6, 71–80 (1992).

    Article  CAS  Google Scholar 

  22. Wenzel, A. et al. EMBO J. 10, 3703–3712 (1991).

    Article  CAS  Google Scholar 

  23. Rustgi, A. K., Dyson, N. & Bernards, R. Nature 352, 541–544 (1991).

    Article  ADS  CAS  Google Scholar 

  24. Van Antwerp, M. E. et al. Proc. natn. Acad. Sci. U.S.A. 89, 9010–9014 (1992).

    Article  ADS  CAS  Google Scholar 

  25. Bernards, R., Dessain, S. K. & Weinberg, R. A. Cell 47, 667–674 (1986).

    Article  CAS  Google Scholar 

  26. Inghirami, G. et al. Science 250, 682–686 (1990).

    Article  ADS  CAS  Google Scholar 

  27. Prendergast, G. C. & Cole, M. D. Molec. cell Biol. 9, 124–134 (1989).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York, 10021, USA

    Ananda L. Roy, Cynthia Carruthers, Thomas Gutjahr & Robert G. Roeder

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  1. Ananda L. Roy
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  2. Cynthia Carruthers
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  3. Thomas Gutjahr
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  4. Robert G. Roeder
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Roy, A., Carruthers, C., Gutjahr, T. et al. Direct role for Myc in transcription initiation mediated by interactions with TFII-I. Nature 365, 359–361 (1993). https://doi.org/10.1038/365359a0

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