Skip to main content
SpringerLink
Log in

Fission yeast wee1 protein kinase is not required for DMA damage-dependent mitotic arrest

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

CHECKPOINTS maintain the dependency relationships between discrete events in the cell cycle (for example, ensuring mitosis does not occur before DNA replication is complete)1,2. In Schizosaccharomyces pombe, mitotic checkpoints monitor DNA synthesis3 and the presence of DNA damage4,5. The replication-dependent mitotic checkpoint prevents mitosis by inactivating p34cdc2 kinase3. The mechanism by which the DNA damage checkpoint interacts with the mitotic machinery is distinct from that used by the replication checkpoint4,5. The activity of p34cdc2 is controlled, in part, by the wee1 protein kinase6, which inactivates cdc2 through phosphorylation at tyrosine-15 (ref. 7). Here we report normal mitotic arrest after DNA damage in S. pombe cells in which the wee1 gene is defective or missing. We suggest why these findings contradict a recent report8 which suggested that the wee1 gene product was required for DNA damage-dependent mitotic arrest.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hartwell, L. H. & Weinert, T. A. Science 246, 629–634 (1989).

    Article  ADS  CAS  Google Scholar 

  2. Enoch, T. & Nurse, P. Cell 65, 921–923 (1991).

    Article  CAS  Google Scholar 

  3. Enoch, T. & Nurse, P. Cell 60, 665–673 (1990).

    Article  CAS  Google Scholar 

  4. Al-Khodairy, F. & Carr, A. M. EMBO J. 11, 1343–1350 (1992).

    Article  CAS  Google Scholar 

  5. Rowley, R., Subramani, S. & Young P. G. EMBO J. 11, 1335–1342 (1992).

    Article  CAS  Google Scholar 

  6. Nurse, P. Nature 344, 503–507 (1990).

    Article  ADS  CAS  Google Scholar 

  7. Featherstone, C. & Russell, P. Nature 349, 808–811 (1991).

    Article  ADS  CAS  Google Scholar 

  8. Rowley, R., Hudson, J. & Young, P. G. Nature 356, 353–355 (1992).

    Article  ADS  CAS  Google Scholar 

  9. Russell, P. & Nurse, P. Cell 49, 559–567 (1987).

    Article  CAS  Google Scholar 

  10. Nurse, P. Nature 256, 547–556 (1975).

    Article  ADS  CAS  Google Scholar 

  11. Mitchison, J. M. & Carter, B. L. A. Meth. Cell Biol. 11, 201–219 (1975).

    Article  CAS  Google Scholar 

  12. Nurse, P. & Bisset, Y. Nature 292, 558–560 (1981).

    Article  ADS  CAS  Google Scholar 

  13. Hagan, I. M. & Hyams, J. S. J. Cell Sci. 89, 343–357 (1988).

    PubMed  Google Scholar 

  14. Hagan, I. M. thesis, Univ. London (1988).

Download references

Author information

Author notes

  1. Nicholas Barbet

    Present address: Biozentrum, University of Basel, Klingeibergstrasse 70, CH4056, Switzerland

  2. Antony Carr: To whom correspondence should be addressed.

Authors and Affiliations

  1. MRC Cell Mutation Unit, Sussex University, Palmer, Sussex, BN19RR, UK

    Nicholas Barbet & Antony Carr

Authors
  1. Nicholas Barbet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Antony Carr
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barbet, N., Carr, A. Fission yeast wee1 protein kinase is not required for DMA damage-dependent mitotic arrest. Nature 364, 824–827 (1993). https://doi.org/10.1038/364824a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/364824a0

  • Springer Nature Limited

This article is cited by

Search

Navigation