Abstract
T LYMPHOCYTES recognize antigens as peptide fragments associated with molecules encoded by the major histocompatibility complex (MHC) and expressed on the surface of antigen-presenting cells1. In the thymus, T cells bearingαβ receptors that react with the MHC molecules expressed by radioresistant stromal elements are positively selected for maturation2–5. In (A × B → A) bone marrow chimaeras, T cells restricted to the MHC-A haplotype are positively selected, whereas MHC-B-reactive thymocytes are not. We investigated whether the introduction of particular thymic stromal elements bearing MHC-B molecules could alter the fate of B-reactive T cells in these (A × B → A) chimaeras. Thymic epithelial cell (TEC) lines expressing H−2b were introduced by intrathymic injection into (H−2b/s → H2S) bone marrow chimaeras and we measured their ability to generate H−2b-restricted cytotoxic T-lymphocytes (CTLs). We report here that one TEC line, 427.1, was able positively to select CTLs specific for influenza and vesicular stomatitis virus antigens in association with class I H−2bmolecules. In addition, line 427.1 can process cytoplasmic proteins for presentation to H−2Kb- and H−2Db-restricted CTLs. Thus, a TEC line capable of normal class I MHC antigen processing and presentation in vitro can induce positive selection after intrathymic injection.
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References
Yewdell, J. W. & Bennink, J. R. Adv. Immun. (in the press).
Bevan, M. J. Nature 269, 417–418 (1977).
Zinkernagel, R. M. et al. J. exp. Med. 147, 882–896 (1978).
Sprent, J. J. exp. Med. 146, 1838–1842 (1978).
Kisielow, P., Teh, H. S., Bluthmann, H. & von Boehmer, H. Nature 335, 730–733 (1988).
Brinster, R. L. et al. Cell 37, 367–379 (1984).
Townsend, A. R. M. et al. Cell 44, 959–968 (1986).
Rotzschke, O. et al. Nature 348, 252–254 (1990).
Van Bleek, G. M. & Nathenson, S. G. Nature 348, 213–216 (1990).
Fiers, W. et al. Nature 273, 113–120 (1978).
Marrack, P. & Kappler, J. Immun. Today 9, 308–315 (1988).
Marrack, P., McCormack, J. & Kappler, J. Nature 338, 503–505 (1989).
Murphy, D. B. et al. Nature 338, 765–768 (1989).
Mizuochi, T., Kasai, M., Kokuho, T., Kakiuchi, T. & Hirokawa, K. J. exp. Med. 175, 1601–1605 (1992).
Nikolic-Zugic, J. & Bevan, M. J. Nature 344, 65–67 (1990).
Sha, W. C. et al. Proc. natn. Acad. Sci. U.S.A. 87, 6186–6190 (1990).
Berg, L. J., Frank, G. D. & Davis, M. M. Cell 60, 1043–1053 (1990).
Henderson, R. A. et al. Science 255, 1264–1266 (1992).
Wei, M. & Cresswell, P. Nature 356, 443–446 (1992).
Powis, S. J. et al. Nature 357, 211–215 (1992).
Townsend, A. et al. Nature 340, 443–448 (1989).
Hosken, N. A. & Bevan, M. J. Science 248, 367–370 (1990).
Spies, T. et al. Nature 355, 644–646 (1992).
Moore, M. W., Carbone, F. R. & Bevan, M. J. Cell 54, 777–785 (1988).
Nuchtern, J. G., Bonifacino, J. S., Biddison, W. E. & Klausner, R. D. Nature 339, 223–226 (1989).
Yewdell, J. W. & Bennink, J. R. Science 244, 1072–1075 (1989).
Grandea, A. G. & Bevan, M. J. Proc. natn. Acad. Sci. U.S.A. 89, 2794–2798 (1992).
Fink, P. Immun. Today 9, 377–380 (1988).
Dennert, G., Hyman, R., Lesley, J. & Trowbridge, I. Cell. Immun. 53, 350–364 (1980).
Goldschneider, I., Komschlies, K. L. & Greiner, D. L. J. exp. Med. 163, 1–17 (1986).
Rotzschke, O. et al. Eur. J. Immun. 21, 2891–2894 (1991).
Nikolic-Zugic, J. & Carbone, F. R. Eur. J. Immun. 20, 2431–2437 (1990).
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Vukmanović, S., Grandea, A., Faas, S. et al. Positive selection of T-lymphocytes induced by intrathymic injection of a thymic epithelial cell line. Nature 359, 729–732 (1992). https://doi.org/10.1038/359729a0
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DOI: https://doi.org/10.1038/359729a0
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