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Antisense c-myb oligonucleotides inhibit intimal arterial smooth muscle cell accumulation in vivo

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Abstract

SYNTHETIC antisense oligonucleotides have been used to dissect gene function in vitro. Technical difficulties prevented the use of this approach for investigating the effect of gene products in vivo. Here we report the use of local delivery of antisense c-myb oligonu-cleotide to suppress intimal accumulation of rat carotid arterial smooth muscle cells. Our results suggest that antisense oligonucleotides can be used to define the in vivo biological role of specific macromolecules in the blood vessel wall and could potentially serve as a new class of therapeutic agents for cardiovascular disorders.

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Author notes

  1. Robert D. Rosenberg: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Michael Simons & Robert D. Rosenberg

  2. Division of Health, Science and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Elazer R. Edelman

  3. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Elazer R. Edelman, Jean-Luc DeKeyser & Robert Langer

  4. Department of Medicine, Harvard Medical School, Beth Israel Hospital, Boston, Massachusetts, 02122, USA

    Michael Simons & Robert D. Rosenberg

Authors
  1. Michael Simons
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  2. Elazer R. Edelman
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  3. Jean-Luc DeKeyser
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  4. Robert Langer
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  5. Robert D. Rosenberg
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Simons, M., Edelman, E., DeKeyser, JL. et al. Antisense c-myb oligonucleotides inhibit intimal arterial smooth muscle cell accumulation in vivo. Nature 359, 67–70 (1992). https://doi.org/10.1038/359067a0

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  • DOI: https://doi.org/10.1038/359067a0

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