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SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity

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Abstract

PROTEIN targeting to the endoplasmic reticulum (ER) in mammalian cells is catalysed by the signal recognition particle (SRP), which consists of six protein subunits and an RNA subunit1,2. Saccharomyces cerevisiae SRP is a 16S particle, of which only two subunits have been identified: a protein subunit, SRP54p, which is homologous to the mammalian SRP54 subunit, and an RNA subunit, scRl (ref. 3). The sec65-lmutant yeast cells4 are temperature-sensitive for growth and defective in the translocation of several secreted and membrane-bound proteins. The DNA sequence of the SEC65 gene suggests that its product is related to mammalian SRP19 subunit and may have a similar function5.Here we show that SEC65p is a subunit of the S. cerevisiae SRP and that it is required for the stable association of another subunit, SRP54p, with SRP. Overexpression of SRP54p suppresses both growth and protein translocation defects insec65-l mutant cells.

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Author notes

  1. Colin J. Stirling: Department of Biochemistry and Molecular Biology, University of Manchester, School of Biological Sciences, Manchester M13 9PT, UK

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, Medical School, San Francisco, California, 94143-0448, USA

    Byron Hann, Colin J. Stirling & Peter Walter

Authors
  1. Byron Hann
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  2. Colin J. Stirling
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  3. Peter Walter
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Hann, B., Stirling, C. & Walter, P. SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity. Nature 356, 532–533 (1992). https://doi.org/10.1038/356532a0

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  • DOI: https://doi.org/10.1038/356532a0

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