Skip to main content
SpringerLink
Log in

Sequence identification of 2,375 human brain genes

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

WE recently described a new approach for the rapid characterization of expressed genes by partial DNA sequencing to generate 'expressed sequence tags'1. From a set of 600 human brain complementary DNA clones, 348 were informative nuclear-encoded messenger RNAs. We have now partially sequenced 2,672 new, independent cDNA clones isolated from four human brain cDNA libraries to generate 2,375 expressed sequence tags to nuclear-encoded genes. These sequences, together with 348 brain expressed sequence tags from our previous study, comprise more than 2,500 new human genes and 870,769 base pairs of DNA sequence. These data represent an approximate doubling of the number of human genes identified by DNA sequencing and may represent as many as 5% of the genes in the human genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adams, M. D. et al. Science 252, 1651–1656 (1991).

    Article  ADS  CAS  Google Scholar 

  2. Ellisen, L. et al. Cell 66, 649–661 (1991).

    Article  CAS  Google Scholar 

  3. Carthew, R. & Rubin, G. Cell 63, 561–577 (1990).

    Article  CAS  Google Scholar 

  4. Rao, Y., Jan, L. & Jan, Y. Nature 345, 163–167 (1990).

    Article  ADS  CAS  Google Scholar 

  5. Woods, D. & Bryant, P. Cell 66, 451–464 (1991).

    Article  CAS  Google Scholar 

  6. Finkelstein, R., Smouse, D. Capaci, T., Spradling, A. & Perrimon, N. Genes Dev. 4, 1516–1527 (1990).

    Article  CAS  Google Scholar 

  7. Uberbacher, E. & Mural, R. Proc. natn. Acad. Sci. U.S.A. 88, 11261–11265 (1991).

    Article  ADS  CAS  Google Scholar 

  8. Kamholtz, J., de Ferra, F., Puckett, C. & Lazzarini, R. Proc. natn. Acad. Sci. U.S.A. 83, 4962–4966 (1986).

    Article  ADS  Google Scholar 

  9. Galau, G., Klein, W., Britten, R. & Davidson, E. Archs Biochem. Biophys. 197, 584–599 (1977).

    Article  Google Scholar 

  10. Casero, R. et al. J. biol. Chem. 266, 810–814 (1991).

    CAS  PubMed  Google Scholar 

  11. Young, M. et al. Genomics 7, 491–502 (1990).

    Article  CAS  Google Scholar 

  12. Höög, C. Nucleic Acids Res. 19, 6123–6127 (1991).

    Article  Google Scholar 

  13. McKusick, V. FASEB J. 5, 12–20 (1991).

    Article  CAS  Google Scholar 

  14. Rubenstein, J. et al. Nucleic Acids Res. 18, 4833–4842.

  15. Schmid, C. W. & Jalinek, W. R. Science 216, 1065–1070 (1982).

    Article  ADS  CAS  Google Scholar 

  16. Paulson, K. E. et al. Nature 316, 359–361 (1985).

    Article  ADS  CAS  Google Scholar 

  17. Fanning, T. G. & Singer, M. F. Biochim biophys. Acta 910, 203–212 (1987).

    Article  CAS  Google Scholar 

  18. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. molec. Biol. 215, 403–410 (1990).

    Article  CAS  Google Scholar 

  19. Barker, W., George, D., Hunt, L. & Garavelli, J. Nucleic Acids Res. 19 (Suppl), 2231–2236 (1991).

    Article  CAS  Google Scholar 

  20. Pearson, P. Nucleic Acids Res. 19 (Suppl), 2237–2239 (1991).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Receptor Biochemistry and Molecular Biology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Mark D. Adams, Mark Dubnick, Anthony R. Kerlavage, Ruben Moreno, Jenny M. Kelley, Teresa R. Utterback, James W. Nagle, Chris Fields & J. Craig Venter

Authors
  1. Mark D. Adams
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mark Dubnick
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anthony R. Kerlavage
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ruben Moreno
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jenny M. Kelley
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Teresa R. Utterback
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. James W. Nagle
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Chris Fields
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. J. Craig Venter
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Adams, M., Dubnick, M., Kerlavage, A. et al. Sequence identification of 2,375 human brain genes. Nature 355, 632–634 (1992). https://doi.org/10.1038/355632a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/355632a0

  • Springer Nature Limited

This article is cited by

Search

Navigation