Abstract
To test models of T-cell recognition, mice transgenic for T-cell receptor α or β chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor Vαor Vβ chains, indicating direct T-cell receptor–peptide contact, and allowing derivation of a topology for the T-cell receptor–MHC interaction. At one position on the peptide, variants transformed a homogeneous Vβ response into a very heterogeneous one.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kronenberg, M., Siu, G., Hood, L. E. & Shastri, N. A. Rev. Immun. 4, 529–591 (1986).
Novotny, J., Tonegawa, S., Saito, H. Kranz, D. M. & Eisen, H. N. Proc. natn. Acad. Sci. U.S.A. 83, 742–746 (1986).
Davis, M. M. & Bjorkman, P. J. Nature 334, 395–402 (1988).
Chothia, C., Boswell, D. R. & Lesk, A. M. EMBO J. 7, 3745–3755 (1988).
Zinkernagel, R. M. & Doherty, P. C. Nature 251, 547–548 (1974).
Saito, T. & Germain, R. N. Nature 329, 256–259 (1987).
Claverie, J. M., Prochnicka-Chalufour, A. & Bougueleret, L. Immun. Today 10, 10–14 (1989).
Hedrick, S. M. Adv. Immun. 43, 193–234 (1988).
Hedrick, S. M. et al. Science 239, 1541–1544 (1988).
Engel, I. & Hedrick, S. M. Cell 54, 473–484 (1988).
Acha-Orbea, H. et al. Cell 54, 263–273 (1988).
Urban, J. L. et al. Cell 54, 577–592 (1988).
Rodewald, H. R., Koszinowski, U. H., Eichmann, K. & Melchers, I. J. Immun. 143, 4238–4243 (1989).
Lai, M.-Z., Jang, Y.-J., Chen, L-K. & Gefter, M. L. J. Immun. 144, 4851–4856 (1990).
Sorger, S. B., Paterson, Y., Fink, P. J. & Hedrick, S. M. J. Immun. 144, 1127–1135 (1990).
Danska, J. S., Livingstone, A. M., Paragas, V., Ishihara, T. & Fathman, C. G. J. Expl Med. 172, 27–33 (1990).
Aebischer, T., Oehen, S. & Hengartner, H. Eur. J. Immun. 20, 523–531 (1990).
Yanagi, Y., Maekawa, R., Cook, T., Kanagawa, O. & Oldstone, M. B. A. J. Virol. 64, 5919–5926 (1990).
Wither, J., Pawling, J., Phillips, L., Delovitch, T. & Hozumi, N. J. Immun. 146, 3513–3522 (1991).
Jarvik, J. & Botstein, D. Proc. natn. Acad. Sci. U.S.A. 72, 2738–2742 (1975).
Botstein, D. & Maurer, R. A. Rev. Genet 16, 61–83 (1982).
Fox, B. S. et al. J. Immun. 139, 1578–1588 (1987).
Schwartz, R. H. et al. J. Immun. 135, 2598–2609 (1985).
Fox, B. S., Carbone, F. R., Germain, R. N., Paterson, Y. & Schwartz, R. H. Nature 331, 538–540 (1988).
Wettstein, D. A., Boniface, J. J., Reay, P. A., Schild, H. & Davis, M. M. J. exp. Med. 174, 219–228 (1991).
Fink, P. J. et al. Nature 321, 219–226 (1986).
Fersht, A. R. et al. Nature 314, 235–238 (1985).
Davies, D. R., Padlan, E. A. & Sheriff, S. A. Rev. Biochem. 59, 439–473 (1990).
Burley, S. K. & Petsko, G. A. FEBS Lett. 203, 139–143 (1986).
Dougherty, D. A. & Stauffer, D. A. Science 250, 1558–1560 (1990).
Ronchese, F., Schwartz, R. H. & Germain, R. N. Nature 329, 254–256 (1987).
Krieger, J. I. et al. J. Immun. 146, 2331–2340 (1991).
Colman, P. M. Adv. Immun. 43, 99–132 (1988).
Ajitkumar, P. et al. Cell 54, 47–56 (1988).
Peccoud, J., Dellabona, P., Allen, P., Benoist, C. & Mathis, D. EMBO J. 9, 4215–4223 (1990).
Huppi, K. E. et al. Immunogenetics 27, 51–56 (1988).
Tjoelker, L. W. et al. Proc. natn. Acad. Sci. U.S.A. 87, 7856–7860 (1990).
Taylor, A. H., Haberman, A. M., Gerhard, W. & Caton, A. J. J. exp. Med. 172, 1643–1651 (1990).
Sarmiento, M., Glasebrook, A. L. & Fitch, F. W. J. Immun. 125, 2665–2672 (1980).
White, J. et al. J. Immun. 143, 1822–1825 (1989).
Rock, K. in Hybridoma Technology in the Biosciences and Medicine (ed. Springer, T. A.) 527–544 (Plenum, New York, 1985).
Chomczynski, P. & Sacchi, N. Analyt. Biochem. 162, 156–159 (1987).
Chen, S. & Evans, G. A. Biotechniques 8, 32–33 (1990).
Casanova, J.-L., Pannetier, C., Jaulin, C. & Kourilsky, P. Nucleic Acids Res. 18, 4028 (1990).
Pullen, A. M., Marrack, P. & Kappler, J. W. Nature 335, 796–801 (1988).
Jameson, S. C. et al. J. Immun. 147, 3185–3193 (1991).
Gascoigne, N. R. J., Chien, Y., Becker, D. M., Kavaler, J. & Davis, M. M. Nature 310, 387–391 6(1984).
Tomonari, K., Lovering, E. & Spencer, S. Immunogenetics 31, 5–6 (1990).
Kanagawa, O., Palmer, E. & Bill, J. Cell. Immun. 119, 412–426 (1989).
Staerz, U., Rammensee, H., Benedetto, J. & Bevan, M. J. Immun. 134, 3994–4000 (1985).
Liao, N.-S., Maltzman, J. & Raulet, D. H. J. exp. Med. 170, 135–143 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jorgensen, J., Esser, U., Fazekas de St. Groth, B. et al. Mapping T-cell receptor–peptide contacts by variant peptide immunization of single-chain transgenics. Nature 355, 224–230 (1992). https://doi.org/10.1038/355224a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/355224a0
- Springer Nature Limited
This article is cited by
-
T cell receptor repertoires associated with control and disease progression following Mycobacterium tuberculosis infection
Nature Medicine (2023)
-
Thymic regulatory T cells arise via two distinct developmental programs
Nature Immunology (2019)
-
On the feasibility of mining CD8+ T cell receptor patterns underlying immunogenic peptide recognition
Immunogenetics (2018)
-
Evolution of innate-like T cells and their selection by MHC class I-like molecules
Immunogenetics (2016)
-
DockTope: a Web-based tool for automated pMHC-I modelling
Scientific Reports (2015)