Abstract
THE major transforming protein of bovine papillomavirus type 1, á£5 (refs 1–4), is mainly associated with endomembranes5, 6, specifically binding to a cellular protein of relative molecular mass 16,000 (16K) (ref. 7). At the same time as transformation, E5 causes the phosphorylation of tyrosine residues in epidermal and platelet-derived growth factor receptors8, 9. We show here that the 16K protein associated with E5 is the 16K component of vacuolar ATPases. This protein is known to be an integral membrane protein in endosomes, bovine chromaffin granules, synaptic vesicles, fungal and plant vacuoles and clathrin-coated vesicles10–16, as well as a component of gap-junction-like membrane complexes17. Because proton pumps are critical for the function of cellular compartments that process growth-factor receptors, the interaction of E5 with the 16K protein could explain the pleiomorphic features of cells transformed by E5.
Similar content being viewed by others
References
Yang, Y.-C., Okayama, H. & Howley, P. M., Proc. natn. Acad. Sci. U.S.A. 82, 1030–1034 (1985).
DiMaio, D., Guralski, D. & Schiller, J. T. Proc. natn. Acad. Sci. U.S.A. 83, 1797–1801 (1986).
Groff, D. & Lancaster, W. D. Virology 150, 221–230 (1986).
Schiller, J. D., Vass, W. C., Vousden, K. H. & Lowy, D. R. J. Virol. 57, 1–6 (1986).
Schlegel, R., Wade-Glass, M., Rabson, M. S. & Yang, Y.-C. Science 233, 464–467 (1986).
Burkhardt, A., Willingham, M., Gay, C., Jeang, K.-T. & Schlegel, R. Virology 170, 334–339 (1989).
Goldstein, D. J. & Schlegel, R. EMBO J. 9, 137–146 (1990).
Martin, P., Vass, W. C., Schiller, J. T., Lowy, D. R. & Velu, T. J. Cell 59, 21–32 (1989).
Petti, L., Nilson, L. & DiMaio, D. EMBO J. 10, 845–855 (1991).
Mandel, M. et al. Proc. natn. Acad. Sci. U.S.A. 85, 5521–5524 (1988).
Arai, H., Berne, M. & Forgac, M. J. biol. Chem. 262, 11006–11011 (1987).
Sun, S.-Z., Xie, X.-S. & Stone, D. K. J. biol. Chem. 262, 14790–14794 (1987).
Bowman, E. J. J. biol. Chem. 258, 15238–15244 (1983).
Uchida, E., Ohsumi, Y. & Anraku, Y. J. biol. Chem. 260, 1090–1095 (1985).
Manolson, M. F., Rea, P. A. & Poole, R. J. J. biol. Chem. 260, 12273–12279 (1985).
Randall, S. K. & Sze, H. J. biol. Chem. 261, 1364–1371 (1986).
Leitch, B. & Finbow, M. E. Expl Cell Res. 190, 218–226 (1990).
Finbow, M. E., Buultjens, T. E. J., Lane, N. J., Shuttleworth, J. & Pitts, J. D. EMBO J. 3, 2271–2278 (1984).
Settleman, J. & DiMaio, D. Proc. natn. Acad Sci. U.S.A. 85, 9007–9011 (1988).
Veillette, A., Horak, I. D. & Bolen, J. B. Oncogene Res. 2, 385–401 (1988).
Buultjens, T. E. J., Finbow, M. E., Lane, N. J. & Pitts, J. D. Cell Tissue Res. 251, 571–580 (1988).
Meagher, L., McLean, P. & Finbow, M. E. Nucleic Acids Res. 18, 22 (1990).
Nelson, H. & Nelson, N. FEBS Lett. 247, 147–153 (1989).
Birman, S. et al. FEBS Lett. 261, 303–306 (1990).
Leavis, P. C., Rosenfeld, S. S., Grabarek, Z., Drabikowski, W. & Gergely, J. J. biol. Chem. 253, 5452–5459 (1978).
Cleveland, D. W., Fischer, S. G., Kirschner, M. W. & Laemmli, U.K. J. biol. Chem. 252, 1102–1106 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Goldstein, D., Fmbow , M., Andresson, T. et al. Bovine papillomavirus E5 oncoprotein binds to the 16K component of vacuolar H+-ATPases. Nature 352, 347–349 (1991). https://doi.org/10.1038/352347a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/352347a0
- Springer Nature Limited
This article is cited by
-
Role of HTLV-1 orf-I encoded proteins in viral transmission and persistence
Retrovirology (2019)
-
Inhibition of MHC‐I by Brucella abortus is an early event during infection and involves EGFR pathway
Immunology & Cell Biology (2017)
-
V-ATPase functions in normal and disease processes
Pflügers Archiv - European Journal of Physiology (2009)
-
The E5 protein of BPV-4 interacts with the heavy chain of MHC class I and irreversibly retains the MHC complex in the Golgi apparatus
Oncogene (2006)
-
Bovine papillomavirus E5 oncoprotein binds to the activated form of the platelet-derived growth factor β receptor in naturally occurring bovine urinary bladder tumours
Oncogene (2006)