Abstract
THE protein-tyrosine kinase activity of the proto-oncogene product p60c-src is negatively regulated by the phosphorylation of a tyrosine residue close to the C terminus, tyrosine 527 (refs 1–11). The phosphorylation might be catalysed by a so-far-unidentified tyrosine kinase, distinct from p60c-src (ref. 7). Recently we purified a protein-tyrosine kinase that specifically phosphorylates tyrosine 527 of p60c-src from neonatal rat brain8,12,13. We have now confirmed the specificity of this enzyme by using a mutant p60c-src that has a phenylalanine instead of tyrosine 527, and cloned a complementary DNA that encodes the enzyme. The enzyme is similar to kinases of the src family in that it has two conserved regions, Src-homology regions 2 and 3, upstream of a tyrosine kinase domain. The amino-acid identity of each region is no more than 47%, however, and the enzyme lacks phosphorylation sites corresponding to tyrosines 416 and 527 of p60c-src and has no myristylation signal. These results suggest that this protein-tyrosine kinase, which might negatively regulate p60c-src, represents a new type of tyrosine kinase.
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References
Cooper, J. A., Gould, K. L., Cartwright, C. A. & Hunter, T. Science 231, 1431–1434 (1986).
Cooper, J. A. & King, C. S. Molec. cell. Biol. 6, 4467–4477 (1986).
Courtneidge, S. A. EMBO J. 4, 1471–1477 (1985).
Kimiecik, T. E. & Shalloway, D. Cell 49, 65–73 (1987).
Piwnica-Worms, H., Saunders, K. B., Roberts, T. M., Smith, A. E. & Cheng, S. H. Cell 49, 75–82 (1987).
Cartwright, C. A., Eckhart, W., Simon, S. & Kaplan, P. L. Cell 49, 83–91 (1987).
Jove, R., Kornbluth, S. & Hanafusa, H. Cell 50, 937–943 (1987).
Okada, M. & Nakagawa, H. J. biol. Chem. 264, 20886–20893 (1989).
Hunter, T. & Sefton, B. M. Proc. natn. Acad. Sci. U.S.A. 77, 1311–1315 (1980).
Kornbluth, S., Jove, R. & Hanafusa, H. Proc. natn. Acad. Sci. U.S.A. 84, 4455–4459 (1987).
Cooper, J. A. & MacAuley, A. Proc. natn. Acad. Sci. U.S.A. 85, 4232–4236 (1988).
Okada, M. & Nakagawa, H. Biochem. biophys. Res. Commun. 154, 796–802 (1988).
Okada, M. & Nakagawa, H. J. Biochem. 104, 297–305 (1988).
Cooper, J. A. & Runge, K. Oncogene Res. 1, 297–310 (1987).
Hanks, S. K., Quinn, A. M. & Hunter, T. Science 241, 42–52 (1988).
Takeya, T. & Hanafusa, H. Cell 32, 881–890 (1983).
Shtivelman, E., Lifshitz, B., Gale, R. P., Roe, B. A. & Canaani, E. Cell 47, 277–284 (1986).
Livneh, E., Glazer, L., Segal, D., Schlessinger, J. & Shilo, B.-Z. Cell 40, 599–607 (1985).
Takahashi, M. & Cooper, G. M. Molec. cell. Biol. 7, 1378–1385 (1987).
Matsushime, H., Wang, L.-H. & Shibuya, M. Molec. cell Biol. 6, 3000–3004 (1986).
Hunter, T. Cell 49, 1–4 (1987).
Sadowski, I., Stone, J. C. & Pawson, T. Molec. cell. Biol. 6, 4396–4408 (1986).
Hirai, H. & Varmus, H. E. Molec. cell. Biol. 10, 1307–1318 (1990).
Mayer, B. J., Hamaguchi, M. & Hanafusa, H. Nature 332, 272–275 (1988).
Stahl, M. L., Ferenz, C. R., Kelleher, K. L., Kriz, R. W. & Knopf, J. L. Nature 332, 269–272 (1988).
Vogel, U. S. et al. Nature 335, 90–93 (1988).
Meisenhelder, J., Suh, P.-G., Rhee, S. G. & Hunter, T. Cell 57, 1109–1122 (1989).
Molloy, C. J. et al. Nature 342, 711–714 (1989).
Schuh, S. M. & Brugge, J. S. Molec. cell. Biol. 8, 2465–2471 (1988).
Cartwright, C. A., Simantov, R., Cowan, W. M., Hunter, T. & Eckhart, W. Proc. natn. Acad. Sci. U.S.A. 85, 3348–3352 (1988).
Veillette, A., Bookman, M. A., Horak, E. M., Samelson, L. E. & Bolen, J. B. Nature 338, 257–259 (1988).
Matsuzaki, H., Nakajima, R., Nishiyama, J., Araki, H. & Oshima, Y. J. Bact. 172, 610–618 (1990).
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Nada, S., Okada, M., MacAuley, A. et al. Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src. Nature 351, 69–72 (1991). https://doi.org/10.1038/351069a0
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DOI: https://doi.org/10.1038/351069a0
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