Abstract
STIMULUS–SECRETION coupling in exocrine glands involves Ca2+ release from intracellular stores1,2. In endoplasmic reticulum vesicle preparations from rat exocrine pancreas, an inositol 1,4,5-trisphosphate(InsP3)-sensitive, as well as an InsP3-insensitive, Ca2+ pool has been characterized3. But Ca2+ channels in the endoplasmic reticulum of rat exocrine pancreas have not been demonstrated at the level of single-channel current. We have now used the patch-clamp technique on endoplasmic reticulum vesicles fused by means of the dehydration–rehydration method4–6. In excised patches, single Ba2+- and Ca2+-selective channels were recorded. The channel activity was markedly voltage-dependent. Caffeine increased channel open-state probability, whereas ruthenium red and Cd2+ blocked single-channel currents. Ryanodine, nifedipine and heparin had no effect on channel activity. The channel activity was not dependent on the free Ca2+ concentration, the presence of InsP3, or pH. We conclude that this calcium channel mediates Ca2+ release from an intracellular store through an InsP3-insensitive mechanism.
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Streb, H., Irvine, R. F., Berridge, M. J. & Schulz, I. Nature 306, 67–69 (1983).
Schulz, I. Am. J. Physiol. 239, G335–G347 (1980).
Thévenod, F. et al. J. Membrane Biol. 109, 173–186 (1989).
Criado, M. & Keller, B. U. FEBS Lett. 224, 172–176 (1987).
Schmid, A., Gögelein, H., Kemmer, T. P. & Schulz, I. J. Membrane Biol. 104, 275–282 (1988).
Schmid, A., Burckhardt, G. & Gögelein, H. J. Membrane Biol. 111, 264–275 (1989).
Bayerdörffer, E. et al. J. Membrane Biol. 81, 69–82 (1984).
Streb, H. et al., J. Membrane Biol. 81, 241–253 (1984).
Rousseau, E., Smith, J. S. & Meissner, G. Am. J. Physiol. 253, C364–C368 (1987).
Smith, J. S. et al. J. gen. Physiol. 92, 1–26 (1988).
Ebrlich, B. E. & Watras, J. Nature 336, 583–586 (1988).
Hosey, M. M. & Lazdunski, M. J. Membrane Biol. 104, 81–105 (1988).
Smith, J. S., Coronado, R. & Meissner, G. J. gen. Physiol. 88, 573–588 (1986).
Rousseau, E. & Meissner, G. Am. J. Physiol. 256, H328–H333 (1989).
Smith, J. S., Coronado, R. & Meissner, G. Nature 316, 446–449 (1985).
Nilius, B., Hess, P., Lansman, J. B. & Tsien, R. W. Nature 316, 443–446 (1985).
Marty, A. & Tan, P. J. Physiol., Lond. 419, 665–687 (1989).
Lipscombe, D. et al. Proc. natn. Acad. Sci. U.S.A. 85, 2398–2402 (1988).
Kostyuk, P. G., Mironov, S. L., Tepikin, A. V. & Belan, P.V. J. Membrane Biol. 110, 11–18 (1989).
Osipchuk, Y. V. et al. EMBO J. 9, 697–704 (1990).
Ghosh, T. K., Mullaney, J. M., Tarazi, F. I. & Gill, D. L. Nature 340, 236–239 (1989).
Gill, D. L., Ghosh, T. K. & Mullaney, J. M. Cell Calcium 10, 363–374 (1989).
Kemmer, T. P. et al. J. biol. Chem. 262, 13,758–13,764 (1987).
Lee, K. S. & Tsien, R. W. J. Physiol., Lond. 354, 253–272 (1984).
Gögelein, H. & Greger, R. Pfluegers Arch. ges. Physiol. 410, 288–295 (1987).
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Schmid, A., Dehlinger-Kremer, M., Schulz, I. et al. Voltage-dependent InsP3- insensitive calcium channels in membranes of pancreatic endoplasmic reticulum vesicles. Nature 346, 374–376 (1990). https://doi.org/10.1038/346374a0
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DOI: https://doi.org/10.1038/346374a0
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