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Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID

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Abstract

The potent transactivation domain of the herpes simplex virion protein VP16 was used as a column ligand for affinity chromatography. VP16 binds strongly and highly selectively to the human and yeast TATA box-binding factors. Our results imply that the principal target for acidic activation domains is the TATA-box factor TFIID.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research and Department of Medical Genetics, University of Toronto, Toronto, Ontario, M5G 1L6, Canada

    Keith F. Stringer, C. James Ingles & Jack Greenblatt

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  1. Keith F. Stringer
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  2. C. James Ingles
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  3. Jack Greenblatt
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Stringer, K., Ingles, C. & Greenblatt, J. Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID. Nature 345, 783–786 (1990). https://doi.org/10.1038/345783a0

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  • DOI: https://doi.org/10.1038/345783a0

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