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Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein

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Abstract

A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of IL-1 to the T-cell/fibroblast form of the IL-1 receptor. Recombinant IRAP specifically inhibited IL-1 bioactivity on ¤ cells and endothelial cells in vitro and was a potent inhibitor of IL-1 induced corticosterone production in vivo

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Authors and Affiliations

  1. Departments of Molecular Biology Research The Upjohn Company, 301 Henrietta Street, Kalamazoo, Michigan, 49007, USA

    D. B. Carter, C.-S. C. Tomich, J. L. Slightom, R. N. McEwan, P. K. W. Harris & L. C. Sieu

  2. Biopolymer Chemistry The Upjohn Company, 301 Henrietta Street, Kalamazoo, Michigan, 49007, USA

    M. R. Deibel Jr, A. W. Yem, G. A. Waszak, H. A. Zurcher-Neely, I. M. Reardon & R. L. Heinrikson

  3. Hypersensitivity Diseases Research The Upjohn Company, 301 Henrietta Street, Kalamazoo, Michigan, 49007, USA

    C. J. Dunn, F. F. Sun, J. G. Chosay, M. M. Hardee, B. M. Taylor & D. E. Tracey

  4. Chemical and Biological Screening The Upjohn Company, 301 Henrietta Street, Kalamazoo, Michigan, 49007, USA

    A. L. Laborde, S. E. Truesdell & J. A. Shelly

  5. Cell Biology, The Upjohn Company, 301 Henrietta Street, Kalamazoo, Michigan, 49007, USA

    A. E. Berger, M. J. Bienkowski & T. E. Eessalu

Authors
  1. D. B. Carter
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  2. M. R. Deibel Jr
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  3. C. J. Dunn
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  4. C.-S. C. Tomich
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  5. A. L. Laborde
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  6. J. L. Slightom
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  7. A. E. Berger
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  8. M. J. Bienkowski
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  9. F. F. Sun
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  10. R. N. McEwan
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  11. P. K. W. Harris
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  12. A. W. Yem
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  13. G. A. Waszak
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  14. J. G. Chosay
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  15. L. C. Sieu
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  16. M. M. Hardee
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  17. H. A. Zurcher-Neely
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  18. I. M. Reardon
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  19. R. L. Heinrikson
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  20. S. E. Truesdell
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  21. J. A. Shelly
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  22. T. E. Eessalu
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  23. B. M. Taylor
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  24. D. E. Tracey
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Carter, D., Deibel , M., Dunn, C. et al. Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein. Nature 344, 633–638 (1990). https://doi.org/10.1038/344633a0

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  • DOI: https://doi.org/10.1038/344633a0

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