Skip to main content

Advertisement

SpringerLink
Log in

Enhanced myogenesis in NCAM-transfected mouse myoblasts

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

THE fusion of mononucleate precursor myoblasts to form the multinucleated skeletal muscle fibre is proceeded by a series of complex cell–cell interactions1–5 but the cell-surface molecules involved in these events have not been characterized. During myogenesis in vivo and in vitro, expression of the neural cell adhesion molecule (NCAM) undergoes an isoform transition that precisely correlates with terminal myoblast differentiation and myotube formation6,7. Altered processing of RNA results in the replacement of the transmembrane NCAM (relative molecular mass, 145,000 (145K)) in proliferating myoblasts by a predominant 125K NCAM form linked to glycosyl phosphatidylinositol in myotubes8,9. We now report that mouse myoblasts transfected to constitutively express the human muscle-specific 125K glycosyl-phosphatidylinositol-linked NCAM isoform more readily fuse to form myotubes. This suggests that NCAM plays a part in myoblast fusion and that the isoform switch may promote this function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bischoff, R. in Cell Surface Reviews, Membrane Fusion Vol. 5 (eds Poste, G. & Nicholson, G. L.) 127–175 (North-Holland, Oxford, 1978).

    Google Scholar 

  2. Wakelam, M. J. O. Biochem. J. 228, 1–12 (1985).

    Article  CAS  Google Scholar 

  3. Gibraltar, D. & Turner, D. C. Devl Biol. 112, 292–307 (1985).

    Article  Google Scholar 

  4. Knudsen, K. A. J. Cell Biol. 101, 891–897 (1985).

    Article  CAS  Google Scholar 

  5. Pizzey, J. A., Jones, G. E. & Walsh, F. S. J. Cell Biol. 107, 2307–2317 (1988).

    Article  CAS  Google Scholar 

  6. Covault, J., Merlie, J. P., Goridis, C. & Sanes, J. R. J. Cell Biol. 102, 731–739 (1986).

    Article  CAS  Google Scholar 

  7. Moore, S. E., Moore, S. E., Thomson, J., Kirkness, V., Dickson, G. & Walsh, F. S. J. Cell Biol. 105, 1377–1386 (1987).

    Article  CAS  Google Scholar 

  8. Dickson, G. et al. Cell 50, 1119–1130 (1987).

    Article  CAS  Google Scholar 

  9. Thomson, J. et al. Genes Dev. 3, 348–357 (1989).

    Article  Google Scholar 

  10. Knudsen, K. A. & Horwitz, A. F. Devl Biol. 58, 329–338 (1977).

    Article  Google Scholar 

  11. Endo, T. & Nadal-Ginard, B. Cell 49, 515–526 (1987).

    Article  CAS  Google Scholar 

  12. Menko, S. A. & Boettiger, D. Cell 51, 51–57 (1987).

    Article  CAS  Google Scholar 

  13. Rutishauser, U., Grumet, M. & Edelman, G. M. J. Cell Biol. 97, 145–152 (1983).

    Article  CAS  Google Scholar 

  14. Bixby, J. L., Pratt, R. S., Lilien, J. & Reichardt, L. F. Proc. natn. Acad. Sci. U.S.A. 84, 2555–2559 (1987).

    Article  ADS  CAS  Google Scholar 

  15. Rieger, F. et al. J. Cell Biol. 107, 707–719 (1988).

    Article  CAS  Google Scholar 

  16. Landmesser, L., Dahm, L., Schultz, K. & Rutishauser, U. Devl Biol. 130, 645–670 (1988).

    Article  CAS  Google Scholar 

  17. Grumet, G. M., Rutishauser, U. & Edelman, G. M. Nature 295, 693–695 (1982).

    Article  ADS  CAS  Google Scholar 

  18. Knudsen, K. A., Smith, L. & McElwee, S. J. Cell Biol. 109, 1779–1786 (1989).

    Article  CAS  Google Scholar 

  19. Yaffe, D. & Saxel, O. Nature 270, 725–727 (1977).

    Article  ADS  CAS  Google Scholar 

  20. Doherty, P. et al. J. Cell Biol. 109, 789–798 (1989).

    Article  CAS  Google Scholar 

  21. Pizzey, J., Rowett, L. H., Barton, C. H., Dickson, G. & Walsh, F. S. J. Cell Biol. 109, 789–798 (1989).

    Article  Google Scholar 

  22. Walsh, F. S., Dickson, G., Moore, S. E. & Barton, C. H. Nature 339, 516 (1989).

    Article  ADS  CAS  Google Scholar 

  23. Patel, K., Rossell, R. J., Moore, S. E., Walsh, F. S. & Kemshead, J. T. Int. J. Cancer 44, 1062–1068 (1989).

    Article  CAS  Google Scholar 

  24. Fraser, S. E., Murray, B. A., Chuong, C.-M. & Edelman, G. M. Proc. natn. Acad. Sci. U.S.A. 81, 4222–4226 (1984).

    Article  ADS  CAS  Google Scholar 

  25. Edelman, G. M., Murray, B. A., Mege, R.-M., Cunningham, B. A. & Gallin, W. J. Proc. natn. Acad. Sci. U.S.A. 84, 8502–8506 (1987).

    Article  ADS  CAS  Google Scholar 

  26. Massague, J., Cheifetz, S., Endo, T. & Nadal-Ginard, B. Proc. natn. Acac. Sci. U.S.A. 83, 8206–8210 (1986).

    Article  ADS  CAS  Google Scholar 

  27. Lathrop, B. K., Thomas, K. & Fraser, L. J. Cell Biol. 101, 2194–2198 (1985).

    Article  CAS  Google Scholar 

  28. Gower, H. J. et al. Cell 55, 955–964 (1988).

    Article  CAS  Google Scholar 

  29. Sudher, P. et al. Science 244, 1158–1162 (1989).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Experimental Pathology, United Medical and Dental Schools, Guy's Hospital, London Bridge, London, SE1 9RT, UK

    George Dickson, Dave Peck, Stephen E. Moore, C. Howard Barton & Frank S. Walsh

Authors
  1. George Dickson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dave Peck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stephen E. Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Howard Barton
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Frank S. Walsh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dickson, G., Peck, D., Moore, S. et al. Enhanced myogenesis in NCAM-transfected mouse myoblasts. Nature 344, 348–351 (1990). https://doi.org/10.1038/344348a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/344348a0

  • Springer Nature Limited

This article is cited by

Search

Navigation