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Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus

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Abstract

A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with anti-snRNP antibodies. These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    Xiang-Dong Fu & Tom Maniatis

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  1. Xiang-Dong Fu
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  2. Tom Maniatis
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Fu, XD., Maniatis, T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343, 437–441 (1990). https://doi.org/10.1038/343437a0

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  • DOI: https://doi.org/10.1038/343437a0

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