Abstract
Kinesin is a motor protein that uses energy derived from ATP hydrolysis to move organelles along microtubules. Using a new technique for measuring the movement produced in vitro by individual kinesin molecules, it is shown that a single kinesin molecule can move a microtubule for several micrometres. New information about the mechanism of force generation by kinesin is presented.
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References
Bagshaw, C. R. Muscle Contraction (Chapman and Hall, London, 1982).
Cooke, R. CRC Crit. Rev. Biochem. 21, 53–118 (1986).
Sheetz, M. P. & Spudich, J. A. Nature 303, 31–35 (1983).
Gelles, J., Schnapp, B. J. & Sheetz, M. P. Nature 331, 450–453 (1988).
Vale, R. D., Reese, T. S. & Sheetz, M. P. Cell 42, 39–50 (1985).
Kron, S. J. & Spudich, J. A. Proc. natn. Acad. Sci. U.S.A. 83, 6272–6276 (1986).
Kishino, A. & Yanagida, T. Nature 334, 74–76 (1988).
Bloom, G. S., Wagner, M. C., Pfister, K. K. & Brady, S. T. Biochemistry 27, 3409–3416 (1988).
Kuznetsov, S. A. et al. EMBO J. 7, 353–356 (1988).
Ingold, A. L., Cohn, S. A. & Scholey, J. M. J. Cell Biol. 107, 2657–2667 (1988).
Hirokawa, N. et al. Cell 56, 867–878 (1989).
Kuznetsov, S. A., Vaisberg, E. A., Rothwell, R. W., Murphy, D. B. & Gelfand, V. I. J. biol. Chem. 264, 589–595 (1989).
Scholey, J. M., Heuser, J., Yang, J. T. & Goldstein, L. S. B. Nature 338, 355–357 (1989).
Yang, J. T., Laymon, R. A. & Goldstein, L. S. B. Cell 56, 879–889 (1989).
Cohn, S. A., Ingold, A. L. & Scholey, J. M. J. biol. Chem. 264, 4290–4297 (1989).
Kron, S. J., Toyoshima, Y. Y. & Spudich, J. A. Biophys. J. 53, 195 (1988).
Vale, R. D., Soll, D. & Gibbons, I. R. Cell (in the press).
Amos, L. A. J. Cell Sci. 87, 105–111 (1987).
Mitchison, T. & Kirschner, M. Nature 312, 237–242 (1984).
Fersht, A. Enzyme Structure and Mechanism (Freeman, New York, 1985).
Brennen, C. & Winet, H. A. Rev. Fluid Mech. 9, 339–398 (1977).
Kuznetsov, S. A. & Gelfand, V. I. Proc. natn. Acad. Sci. U.S.A. 83, 8530–8534 (1986).
Hackney, D. D. Proc. natn. Acad. Sci. U.S.A. 85, 6314–6318 (1988).
Toyoshima, Y. Y. et al. Nature 328, 536–539 (1987).
Harada, Y., Noguchi, A., Kishino, A. & Yanagida, T. Nature 326, 805–808 (1987).
Sale, W. S. & Fox, L. A. J. Cell Biol. 107, 1793–1797 (1988).
Miller, R. H. & Lasek, R. J. J. Cell Biol. 101, 2181–2193 (1985).
Neher, E. & Sakmann, B. Nature 260, 799–802 (1976).
Weingarten, M. D., Suter, M. M., Littman, D. R. & Kirschner, M. W. Biochemistry 13, 5529–5537 (1974).
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Howard, J., Hudspeth, A. & Vale, R. Movement of microtubules by single kinesin molecules. Nature 342, 154–158 (1989). https://doi.org/10.1038/342154a0
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DOI: https://doi.org/10.1038/342154a0
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