Mycoplasma contamination is tough to detect and even more difficult to eradicate. It is best to start over fresh from clean cell stocks, but several elimination options are available.
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Hay, R., Macy, M. & Chen, T. Mycoplasma infection of cultured cells. Nature 339, 487–488 (1989). https://doi.org/10.1038/339487a0
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DOI: https://doi.org/10.1038/339487a0
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