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Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein

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Abstract

AN N-ethylmaleimide-sensitive fusion protein (NSF) has been purified on the basis of its ability to catalyse vesicular transport within the Golgi stack. We report here that this same protein is required for transport from the endoplasmic reticulum to the Golgi stack in semi-intact cells. This transport process is inhibited by a monoclonal antibody against NSF. Furthermore, pretreatment of semi-intact cells with N -ethylmaleimide, a sulphydryl alkylating reagent, inhibits transport. Addition of highly purified NSF largely restores transport from endoplasmic reticulum to Golgi. These results suggest that NSF is a general component of the transport machinery required for membrane fusion at multiple stages of the secretory pathway.

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Author notes

  1. Marc R. Block

    Present address: Laboratoire de Physiologic Cellulaire, Universite Joseph Fourier, B.P. 53X, 38041, Grenoble Cedex, France

  2. Benjamin S. Glick

    Present address: Biocenter, University of Basel, Klingelbergstraße 70, CH-4056, Basel, Switzerland

Authors and Affiliations

  1. Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, California, 92037, USA

    Con J. M. Beckers & William E. Balch

  2. Department of Biochemistry, Stanford University School of Medicine, Stanford, California, 94305, USA

    Marc R. Block & Benjamin S. Glick

  3. Department of Biology, Lewis Thomas Laboratory, Princeton University, Princeton, New Jersey, 08544, USA

    James E. Rothman

  4. Biocenter, University of Basel, Klingelbergstraße 70, CH-4056, Basel, Switzerland

    Marc R. Block

Authors
  1. Con J. M. Beckers
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  2. Marc R. Block
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  3. Benjamin S. Glick
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  4. James E. Rothman
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  5. William E. Balch
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Beckers, C., Block, M., Glick, B. et al. Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein. Nature 339, 397–398 (1989). https://doi.org/10.1038/339397a0

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  • DOI: https://doi.org/10.1038/339397a0

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