Skip to main content
SpringerLink
Log in

Identification of angiogenic activity and the cloning and expression of platelet-derived endothelial cell growth factor

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Cloning and sequencing of the complementary DNA for platelet-derived endothelial cell growth factor indicates that it is a novel factor distinct from previously characterized proteins. The factor, a protein with a relative molecular mass of about 45,000, stimulates endothelial cell growth and chemotaxis in vitro and angiogenesis in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Miyazono, K., Okabe, T., Urabe, A., Takaku, F. & Heldin, C.-H. J. biol. Chem. 262, 4,098–4,103 (1987).

    CAS  Google Scholar 

  2. Miyazono, K. & Heldin, C.-H. Biochemistry 28, 1,704–1,710 (1989).

    Article  CAS  Google Scholar 

  3. Lobb, R. R., Harper, J. W. & Fett, J. W. Analyt. Biochem. 154, 1–14 (1986).

    Article  CAS  Google Scholar 

  4. Gospodarowicz, D., Neufeld, G. & Schweigerer, L. Molec. cell. Endocr. 46, 187–204 (1986).

    Article  Google Scholar 

  5. Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).

    Article  CAS  Google Scholar 

  6. Fiddes, J. C. & Goodman, H. M. Nature 286, 684–687 (1980).

    Article  ADS  CAS  Google Scholar 

  7. Alien, G. in Sequencing of Proteins and Peptides (eds Work, T. S. & Burdon, R. H.) (Elsevier, Amsterdam, 1981).

    Google Scholar 

  8. Piwnica-Worms, H. et al. Molec cell. Biol. 6, 2,033–2,040 (1986).

    Article  CAS  Google Scholar 

  9. Jaye, M. et al. Science 233, 541–545 (1986).

    Article  ADS  CAS  Google Scholar 

  10. Abraham, J. A. et al. EMBO J. 5, 2,523–2,528 (1986).

    Article  CAS  Google Scholar 

  11. Fasano, O., Birnhaum, D., Edlung, L., L., Fogh, J. & Wigler, M. Molec. cell. Biol. 4, 1,695–1,705 (1984).

    Article  CAS  Google Scholar 

  12. Folkman, J. & Klagsbrun, M. Science 235, 442–447 (1987).

    Article  ADS  CAS  Google Scholar 

  13. Schreiber, A. B., Winkler, M. E. & Derynck, R. Science 232, 1,250–1,253 (1986).

    Article  CAS  Google Scholar 

  14. Roberts, A. B. et al. Proc. natn. Acad. Sci. U.S.A. 84, 4,167–4,171 (1986).

    Article  Google Scholar 

  15. Wiseman, D. M., Polverini, P. J., Kamp, D. W. & Leibovich, S. J. Biochem. biophys. Res. Commun. 157, 793–800 (1988).

    Article  CAS  Google Scholar 

  16. Frater-Schröder, M., Risau, W., Hallmann, R., Gautschi, P. & Böhlen, P. Proc. natn. Acad. Sci. U.S.A. 84, 5,277–5,281 (1987).

    Article  Google Scholar 

  17. Leibovich, S. J. et al. Nature 329, 630–632 (1987).

    Article  ADS  CAS  Google Scholar 

  18. Fett, J. W. et al. Biochemistry 24, 5,480–5,486 (1985).

    Article  CAS  Google Scholar 

  19. Ross, R. New Engl. J. Med. 314, 488–500 (1986).

    Article  CAS  Google Scholar 

  20. Ross, R., Raines, E. E. & Bowen-Pope, D. F. Cell 46, 155–159 (1986).

    Article  CAS  Google Scholar 

  21. Han, J. H., Stratowa, C. & Rutter, W. J. Biochemistry 26, 1,617–1,625 (1987).

    Article  CAS  Google Scholar 

  22. Watson, C. J. & Jackson, J. F. in DNA Cloning Vol. 1 (ed. Glover, D. M.) 79–88 (IRL, Oxford, 1985).

    Google Scholar 

  23. Lathe, R. J. molec. Biol. 183, 1–12 (1985).

    Article  CAS  Google Scholar 

  24. Ullrich, A., Berman, C. H., Dull, T. J., Gray, A. & Lee, J. M. EMBO J. 3, 361–364 (1984).

    Article  CAS  Google Scholar 

  25. Yanisch-Perron, C., Veira, J. & Messing, J. Gene 33, 103–119 (1985).

    Article  CAS  Google Scholar 

  26. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5,463–5,467 (1977).

    Article  CAS  Google Scholar 

  27. Maxam, W. W. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  28. Blobel, G. & Dobberstein, B. J. cell. Biol. 67, 835–851 (1975).

    Article  CAS  Google Scholar 

  29. Terracio, L. et al. J. cell. Biol. 107, 1,947–1,957 (1988).

    Article  CAS  Google Scholar 

  30. Zerwes, H.-G. & Risau, W. J. cell. Biol. 105, 2,037–2,041 (1987).

    Article  CAS  Google Scholar 

  31. Risau, W. Proc. natn. Acad. Sci. U.S.A. 83, 3,855–3,859 (1986).

    Article  CAS  Google Scholar 

  32. Crum, R., Szabo, S. & Folkman, J. Science 230, 1,375–1,377 (1985).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Carl-Henrik Heldin: To whom correspondence should be addressed.

Authors and Affiliations

  1. The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113, Japan

    Fuyuki Ishikawa, Kohei Miyazono, Koichi Hagiwara & Fumimaro Takaku

  2. Ludwig Institute for Cancer Research, Biomedical Center, Box 595, S-751 23, Uppsala, Sweden

    Kohei Miyazono, Ulf Hellman, Christer Wernstedt, Kensuke Usuki & Carl-Henrik Heldin

  3. Max-Planck-lnstitute für Psychiatric, Am Klopferspitz ISA, D-8033, Martinsried, FRG

    Hannes Drexler & Werner Risau

Authors
  1. Fuyuki Ishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kohei Miyazono
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ulf Hellman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hannes Drexler
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Christer Wernstedt
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Koichi Hagiwara
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kensuke Usuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Fumimaro Takaku
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Werner Risau
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Carl-Henrik Heldin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ishikawa, F., Miyazono, K., Hellman, U. et al. Identification of angiogenic activity and the cloning and expression of platelet-derived endothelial cell growth factor. Nature 338, 557–562 (1989). https://doi.org/10.1038/338557a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/338557a0

  • Springer Nature Limited

This article is cited by

Search

Navigation