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Lymphocyte activation by HIV-1 envelope glycoprotein

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Abstract

Cell activation by phytohaemagglutinin, phorbol ester and by the supernatant of phytohaemagglutinin-stimulated peripheral blood mononuclear cells induces the expression and cytopathic effects of latent human immunodeficiency virus type-1 (HIV-1) in vitro1–3. The lymphocyte surface protein CD4 has been identified as a receptor for HIV-14,5 and binds the viral envelope glycoprotein (gp120)6,7. In the light of evidence indicating that one natural function of CD4 is as a growth factor receptor8–10, we examined the ability of native gp120 to activate resting CD4-bearing lymphocytes. Our results indicate that gp120 has innate biological activity as a result of a specific interaction with CD4, inducing increases in intracellular levels of inositol trisphosphate and of calcium, and in interleukin-2 receptor expression and cell motility.

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Authors and Affiliations

  1. Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts, 02118, USA

    Hardy Kornfeld, William W. Cruikshank, Jeffrey S. Berman & David M. Center

  2. Program Resources, National Cancer Institute—Frederick Cancer Research Center, Frederick, Maryland, 21701, USA

    Stephen W. Pyle

Authors
  1. Hardy Kornfeld
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  2. William W. Cruikshank
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  3. Stephen W. Pyle
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  4. Jeffrey S. Berman
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  5. David M. Center
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Kornfeld, H., Cruikshank, W., Pyle, S. et al. Lymphocyte activation by HIV-1 envelope glycoprotein. Nature 335, 445–448 (1988). https://doi.org/10.1038/335445a0

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