Abstract
AIDS is a disorder characterized by a slow progressive impairment of immune function and by infection of human immunodeficiency viruses (HIV-1, HIV-2)1–4. Our knowledge of how these viruses cause disease in man, or how the related lentiviruses (visna and equine infectious anaemia virus) cause disease in animals, is still fragmentary. In particular, the significance of genetic variation in HIV-1, occurring within populations, within individuals and over periods of time5,6, and the mechanisms of viral persistence remain unclear. To address these issues we prepared a series of proviral clones of HIV-1 originating from a single patient and compared their biological properties. Here we show that hybrid genomes (in which the envelope region of six viral clones were separately substituted into a prototype HIV-1 genome) generated viruses with widely differing capacity to grow in human T cells, cell lines and monocytoid cultures. These data suggest that extensive biological variation exists in vivo within an infected individual and is in part determined at the level of the viral envelope.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gottlieb, M. S. et al. New Engl. J. Med. 305, 1424–1431 (1981).
Gallo, R. C. et al. Science 224, 500–502 (1984).
Barre-Sinoussi, F. et al. Science 220, 868–871 (1983).
Clavel, F. et al. Science 233, 343–346 (1986).
Shaw, G. M. et al. Science 226, 1165–1171 (1984).
Hahn, B. H. et al. Nature 330, 184–186 (1987).
Hahn, B. et al. Science 232, 1548–1553 (1986).
Saag, M. et al. Nature (this issue).
Fisher, A. G. et al. Nature 320, 367–371 (1986).
Gartner, S., Markovitz, P., Markovitz, D. M., Betts, R. F. & Popovic, M. Science 233, 215–219 (1986).
Overbaugh, J., Donahue, P. R., Quackenbush, S. L., Hover, E. A. & Mullins, J. I. Science 239, 906–910 (1988).
Ratner, L. et al. Aids Res. hum. Retroviruses 3, 57–69 (1987).
Robertson, J. S. et al. Virology 160, 31–37 (1987).
Katz, J. M., Naeve, C. W. & Webster, R. G. Virology 156, 386–395 (1987).
Haase, A. T., Stowring, L., Narayan, O., Griffin, D. & Price, D. Science 195, 175–177 (1977).
Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).
Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).
Rho, H. M., Poiez, B., Ruscetti, F. & Gallo, R. C. Virology 112, 355–360 (1981).
Veronese di Marzo, F. et al. Proc. natn. Acad. Sci. U.S.A. 82, 5199–5205 (1985).
Fisher, A. G. et al. Science 237, 888–893 (1987).
Barin, F. et al. Science 228, 1094–1098 (1985).
Kleinerman, E. S. et al. Cancer Res. 43, 2010–2014 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fisher, A., Ensoli, B., Looney, D. et al. Biologically diverse molecular variants within a single HIV-1 isolate. Nature 334, 444–447 (1988). https://doi.org/10.1038/334444a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/334444a0
- Springer Nature Limited
This article is cited by
-
Threshold virus dynamics with impulsive antiretroviral drug effects
Journal of Mathematical Biology (2012)
-
Massively parallel pyrosequencing highlights minority variants in the HIV-1 env quasispecies deriving from lymphomonocyte sub-populations
Retrovirology (2009)
-
Identification of a major co-receptor for primary isolates of HIV-1
Nature (1996)
-
Genetic variation in populations of kennedya yellow mosaic tymovirus
Archives of Virology (1996)
-
Quasispecies and naturally occurring superinfection in feline immunodeficiency virus infection
Archives of Virology (1996)