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ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM

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Abstract

Antigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens1,2. The LFA-1 antigen is widely expressed on cells of haematopoietic origin and is a major receptor of T cells, B cells and granulocytes3. LFA-1 mediates the leukocyte adhesion reactions underlying cytolytic conjugate formation, helper T-cell interactions, and antibody-dependent killing by natural killer cells and granulocytes. Recently, ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-14–6. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells and disrupt the interaction between cytotoxic T cells and target cells. In addition, purified ICAM-1 reconstituted into artificial membranes binds LFA-1+ cells6. ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. LFA-1 has been placed in the integrin family of cell surface receptors by virtue of the high sequence similarity between the LFA-1 and integrin β chains7,8. The adhesion ligands of the integrin family are gly-coproteins bearing the Arg-Gly-Asp (RGD) sequence motif, for example, fibronectin, fibrinogen, vitronectin and von Willebrand factor9. Here we show that a complementary DNA clone ICAM-1 contains no RGD motifs, but instead is homologous to the neural cell adhesion molecule NCAM10,11.

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Author notes

  1. Malegapuru W. Makgoba: Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 02115, USA

Authors and Affiliations

  1. Department of Molecular Biology and Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, 02114, USA

    David Simmons, Malegapuru W. Makgoba & Brian Seed

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  1. David Simmons
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  2. Malegapuru W. Makgoba
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Simmons, D., Makgoba, M. & Seed, B. ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM. Nature 331, 624–627 (1988). https://doi.org/10.1038/331624a0

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  • DOI: https://doi.org/10.1038/331624a0

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