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Mouse p53 inhibits SV40 origin-dependent DNA replication

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Abstract

p53 is a cellular phosphoprotein that is present at elevated concentrations in cells transformed by different agents1–5. p53 complementary DNA expression-constructs immortalize primary cells in vitro6,7 and co-operate with an activated ras oncogene in malignant transformation6,8,9. Several reports have implicated p53 in mammalian cell cycle control and specifically with events occurring at the G0–G1 boundary10,11. p53 forms specific complexes with simian virus 40 (SV40) large-T antigen1,2, and such complexes are found associated with both replicating and mature SV40 DNA in lytically infected cells12. In an accompanying paper Gannon and Lane13 report that in in vitro plate-binding assays, mouse p53 can displace polymerase α from complex with T-antigen. We have examined the in vivo consequences of expressing wild-type and mutant p53 proteins from other species in SV40-transformed monkey cells. We report here that expression of mouse p53 results in a substantial and selective inhibition of SV40 origin-dependent DNA replication. In addition to any function in the G0-G1 transition, the data presented suggest that p53 may affect directly the initiation or maintenance of replicative DNA synthesis.

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References

  1. Lane, D. P. & Crawford, L. V. Nature 278, 261–263 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Linzer, D. I. H. & Levine, A. J. Cell 17, 43–52 (1979).

    Article  CAS  PubMed  Google Scholar 

  3. DeLeo, A. B. et al. Proc. natn. Acad. Sci. U.S.A. 76, 2420–2424 (1979).

    Article  ADS  CAS  Google Scholar 

  4. Rotter, V., Boss, M. A. & Baltimore, D. J. Virol 38, 336–346 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Ruscetti, S. K. & Scolnick, E. M. J. Virol. 46, 1022–1026 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Jenkins, J. R., Rudge, K. & Currie, G. A. Nature 312, 651–654 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Jenkins, J. R., Rudge, K., Chumakov, P. & Currie, G. A. Nature 317, 816–818 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Eliyahu, D., Raz, A., Gruss, P., Givol, D. & Oren, M. Nature 321, 646–649 (1984).

    Article  ADS  Google Scholar 

  9. Parada, L. F., Land, H., Weinbereg, R. A., Wolf, D. & Rotter, V. Nature 312, 649–651 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Mercer, W. E., Nelson, D., DeLeo, A. B., Old, L. J. & Baserga, R. Proc. natn. Acad. Sci. U.S.A. 79, 6309–6312 (1982).

    Article  ADS  CAS  Google Scholar 

  11. Reich, N. C. & Levine, A. J. Nature 308, 199–201 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Tack, L. C., Wright, J. H. & Gurney, E. G. J. Virol. 58, 635–646 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Gannon, J. V. & Lane, D. P. Nature 329, 456–458 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  14. Cullen, B. R. Cell 46, 973–982 (1986).

    Article  CAS  PubMed  Google Scholar 

  15. Gluzman, Y. Cell 23, 175–182 (1981).

    Article  CAS  PubMed  Google Scholar 

  16. Li, J. J. & Kelly, T. J. Molec. cell. Biol. 5, 1238–1246.

  17. Stillman, B. W. & Gluzman, Y. Molec. cell. Biol. 5, 2051–2060.

  18. Yewdell, J. W., Gannon, J. V. & Lane, D. P. J. Virol. 59, 444–452 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Dippold, W. G., Jay, G., DeLeo, A. B., Khoury, G. & Old, L. J. Proc. natn. Acad. Sci. U.S.A. 78, 1695–1699 (1981).

    Article  ADS  CAS  Google Scholar 

  20. Jenkins, J. R., Rudge, K., Redmond, S. & Wade-Evans, A. Nucleic Acids Res. 12, 5609–5626 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Southern, P. J. & Berg, P. J. molec. appl. Genet. 1, 327–341 (1982).

    CAS  Google Scholar 

  22. Traganos, F., Darzynkiewicz, Z., Sharpless, T. & Melamed, M. R. J. Histochem. Cytochem. 25, 46–56 (1977).

    Article  CAS  PubMed  Google Scholar 

  23. Murray, J. D., Bellett, A. J. D., Braithwaite, A. W., Waldron, L. K. & Taylor, I. W. J. cell. Physiol. 111, 89–97 (1982).

    Article  CAS  PubMed  Google Scholar 

  24. Sturzbecher, H.-W., Chumakov, P., Welch, W. J. & Jenkins, J. R. Oncogene 1, 201–211 (1987).

    CAS  PubMed  Google Scholar 

  25. Wade-Evans, A. thesis, Univ. of London (1986).

  26. Campbell, J. L. A. Rev. Biochem. 55, 733–771 (1986).

    Article  CAS  Google Scholar 

  27. Smale, S. T. & Tjian, R. Molec. cell. Biol. 6, 4077–4087 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Tooze, J. (ed.) DNA Tumor Viruses (Cold Spring Harbor, N.Y., 1980).

  29. Murakami, Y., Wobbe, C. R., Dean, F. B., Weissbach, L. & Hurwitz, J. in Cancer Cells Vol. 4 (Cold Spring Harbor, N.Y., 1986).

    Google Scholar 

  30. Hirt, B. J. molec. Biol. 26, 365–369 (1967).

    Article  CAS  PubMed  Google Scholar 

  31. Hanahan, D. J. molec. Biol. 166, 557–580 (1983).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Marie Curie Research Institute, The Chart, Oxted, Surrey, RH8 OTL, UK

    Antony W. Braithwaite, Horst-Werner Sturzbecher, Christine Addison, Colin Palmer, Karran Rudge & John R. Jenkins

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  1. Antony W. Braithwaite
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  2. Horst-Werner Sturzbecher
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  3. Christine Addison
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  4. Colin Palmer
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  5. Karran Rudge
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  6. John R. Jenkins
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Braithwaite, A., Sturzbecher, HW., Addison, C. et al. Mouse p53 inhibits SV40 origin-dependent DNA replication. Nature 329, 458–460 (1987). https://doi.org/10.1038/329458a0

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