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Discrimination between antibodies to HIV and to related retroviruses using site-directed serology

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Abstract

Human immunodeficiency virus (HIV) strains can be separated into two types: HIV and HIV-related West African viruses. Site-directed serology using synthetic peptides offers possibilities for the determination of type-specific antibodies. A 22-amino-acid peptide with the sequence Ala-Ile-Glu-Lys-Tyr-Leu-Glu-Asp-Gln-Ala-Gln-Leu-Asn-Ala-Trp-Cys-Ala-Phe-Arg-Gln-Val-Cys representing a conserved region of the transmembranous protein of simian T-cell lymphotropic virus-type III (STLV-III; related to West African HIV) was used as antigen in an enzyme-linked immunosorbent assay (ELISA). In parallel, tests were performed with a pair of previously described peptides1,2, including the homologous region of the glycoprotein (gp) 41 of the HIV strain HTLV-IIIB. In tests with three groups of 20 sera it was shown that the different peptide ELISAs allowed a categorical distinction of antibodies to the two types of HIV. Tests using peptide antigens may provide excellent opportunities for large-scale testing for type-specific antibodies against HIV. The tests are simple, sensitive and specific and are readily standardized.

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Authors and Affiliations

  1. Department of Virology, Karolinska Institutet, School of Medicine, c/o SBL S-105 21 Stockholm, Sweden

    Erling Norrby, Francesca Chiodi & Agneta von Gegerfeldt

  2. Department of Immunology, National Bacteriological Laboratory, 105 21, Stockholm, Sweden

    Gunnel Biberfeld

  3. National Laboratory of Public Health, Bissau, Guinea Bissau

    Anders Nauclér

  4. Johnson & Johnson Biotechnology Center, La Jolla, California, 92038, USA

    Elliot Parks

  5. Department of Molecular Virology, Research Institute of Scripps Clinic, La Jolla, California, 92037, USA

    Richard Lerner

Authors
  1. Erling Norrby
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  2. Gunnel Biberfeld
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  3. Francesca Chiodi
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  4. Agneta von Gegerfeldt
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  5. Anders Nauclér
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  6. Elliot Parks
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  7. Richard Lerner
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Norrby, E., Biberfeld, G., Chiodi, F. et al. Discrimination between antibodies to HIV and to related retroviruses using site-directed serology. Nature 329, 248–250 (1987). https://doi.org/10.1038/329248a0

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  • DOI: https://doi.org/10.1038/329248a0

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