Abstract
Plasma membrane receptors for hormones, drugs, neurotransmit-ters and sensory stimuli are coupled to guanine nucleotide regulatory proteins. Recent cloning of the genes and/or cDNAs for several of these receptors including the visual pigment rhodopsin1, the adenylate-cyclase stimulatory β-adrenergic receptor2–4 and two subtypes of muscarinic cholinergic receptors5,6 has suggested that these are homologous proteins with several conserved structural and functional features. Whereas the rhodopsin gene consists of five exons interrupted by four introns1, surprisingly the human and hamster β-adrenergic receptor genes contain no introns in either their coding or untranslated sequences7. We have cloned and sequenced a DNA fragment in the human genome which cross-hybridizes with a full-length β2-adrenergic receptor probe at reduced stringency. Like the β2-adrenergic receptor this gene appears to be intronless, containing an uninterrupted long open reading frame which encodes a putative protein with all the expected structural features of a G-protein-coupled receptor.
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Kobilka, B., Frielle, T., Collins, S. et al. An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins . Nature 329, 75–79 (1987). https://doi.org/10.1038/329075a0
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DOI: https://doi.org/10.1038/329075a0
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