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Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family

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Abstract

Amino-acid sequences derived from complementary DMAs encoding the α- and β-subunits of the GAB A/ benzo diazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the in vitro-generated α-subunit and β-subunit RNAs in Xenopus oocytes produces a functional receptor and ion channel with the pharmacological properties characteristic of the GABAA receptor.

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Author notes

  1. Peter R. Schofield, David R. Burt & Peter H. Seeburg

    Present address: Laboratory of Molecular Neuroendocrinology, ZMBH, Universität Heidelberg, Im Neuenheimer Feld 282, D-6900, Heidelberg, FRG

  2. David R. Burt

    Present address: Department of Pharmacology, University of Maryland School of Medicine, 655 West Baltimore Street, Baltimore, Maryland, 21201, USA

  3. Eric A. Barnard: To whom correspondence should be addressed.

Authors and Affiliations

  1. Genentech, Inc., Department of Developmental Biology, 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    Peter R. Schofield, Henry Rodriguez, Lucy M. Rhee, J. Ramachandran & Peter H. Seeburg

  2. MRC Molecular Neurobiology Unit, MRC Centre, Hills Road, Cambridge, CB2 2QH, UK

    Peter R. Schofield, Mark G. Darlison, Norihisa Fujita, David R. Burt, F. Anne Stephenson, Vincenzina Reale, Thora A. Glencorse & Eric A. Barnard

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  1. Peter R. Schofield
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  2. Mark G. Darlison
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Schofield, P., Darlison, M., Fujita, N. et al. Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family. Nature 328, 221–227 (1987). https://doi.org/10.1038/328221a0

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