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Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes

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Abstract

In human T lymphocytes the antigen receptor (Ti) is associated non-covalently on the cell surface with the invariant T3 antigen which comprises 3 chains: two glycosylated polypeptides of relative molecular mass 26,000 (Mr26K) and 21K (γ and δ) and one non-N-glycosylated polypeptide of Mr 19K (ε)1–7. The proposed function of T3 is to transduce the activation signals delivered via the antigen receptor8–13. Recently we have shown that phorbol esters, which stimulate protein kinase C, can induce phosphorylation of the γ subunit of the T3 antigen14,15. But the critical question is whether T3 phosphorylation occurs as a normal consequence of immune activation of T lymphocytes. In this respect, it has been shown that immune stimulation of murine T cells results in phosphorylation of Ti-associated polypeptides that may be the functional analogues of the human T3 antigen16,17. We have therefore monitored T3 phosphorylation after exposure of human T cells to antigen or phytohaemagglutinin (PHA). The data show that both stimuli initiate phosphorylation of the γ subunit of the T3 antigen which indicates that T3 phosphorylation is a physiological response to immune activation.

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References

  1. Meuer, S. C. et al. J. exp. Med. 157, 705–719 (1983).

    Article  CAS  Google Scholar 

  2. Acuto, O. et al. Cell 34, 717 (1983).

    Article  CAS  Google Scholar 

  3. Meuer, S. C. et al. Science 222, 1239–1241 (1983).

    Article  ADS  CAS  Google Scholar 

  4. Meuer, S. C. et al. Nature 303, 808–810 (1983).

    Article  ADS  CAS  Google Scholar 

  5. Brenner, M. B., Trowbridge, J. S. & Strominger, J. L. Cell 40, 183–190 (1985).

    Article  CAS  Google Scholar 

  6. Kanellopoulus, J. M., Wigglesworth, N. M., Owen, M. J. & Crumpton, M. J. EMBO J. 2, 1807–1814 (1983).

    Article  Google Scholar 

  7. Borst, J., Alexander, S., Elder, J. & Terhorst, C. J. biol. Chem. 258, 5135–5141 (1983).

    CAS  Google Scholar 

  8. Van Wauve, J. P., De Mey, J. R. & Goosens, J. G. J. Immun. 125, 2708–2712 (1980).

    Google Scholar 

  9. Chang, T. W., Kung, P. C., Gingrus, S. P. & Goldstein, G. Proc. natn. Acad. Sci. U.S.A. 78, 1805–1808 (1981).

    Article  ADS  CAS  Google Scholar 

  10. Burns, G. R., Boyd, A. W. & Beverley, P. C. L. J. Immun. 129, 1451–1457 (1982).

    CAS  Google Scholar 

  11. Reinherz, E. L. et al. Cell 30, 735–743 (1982).

    Article  CAS  Google Scholar 

  12. Oettgen, H. C., Terhost, C., Cantley, L. C. & Roscoff, P. M. Cell 40, 583–590 (1985).

    Article  CAS  Google Scholar 

  13. Roscoff, P. M. & Cantley, L. W. J. biol. Chem. 260, 14053–14059 (1985).

    Google Scholar 

  14. Cantrell, D. A., Davies, A. A. & Crumpton, M. J. Proc. natn. Acad. Sci. U.S.A. 82, 8158–8162 (1985).

    Article  ADS  CAS  Google Scholar 

  15. Davies, A. A., Cantrell, D. A. & Crumpton, M. J. Biosciences 5, 867–876 (1985).

    CAS  Google Scholar 

  16. Samelson, L. E., Harford, J. B., Schwartz, N. H. & Klausner, R. D. Proc. natn. Acad. Sci. U.S.A. 82, 1969–1973 (1985).

    Article  ADS  CAS  Google Scholar 

  17. Samelson, L. E., Harford, J. B. & Klausner, R. D. Cell 43, 223–231 (1985).

    Article  CAS  Google Scholar 

  18. Beverley, P. C. L. & Callard, R. E. Eur. J. Immun. 11, 329–334 (1981).

    Article  CAS  Google Scholar 

  19. Walsh, F. S. & Crumpton, M. J. Nature 269, 307–311 (1977).

    Article  ADS  CAS  Google Scholar 

  20. Barnstable, C. J. et al. Cell 14, 9–20 (1978).

    Article  CAS  Google Scholar 

  21. Chilson, O. P., Boylston, A. W. & Crumpton, M. J. EMBO J. 3239–3245 (1984).

  22. O'Flynn, K., Krensky, A. M., Beverley, P. C. L., Burakoff, S. J. & Lynch, D. C. Nature 313, 686–687 (1985).

    Article  ADS  CAS  Google Scholar 

  23. Ohashi, P. S. et al. Nature 316, 606–609 (1985).

    Article  ADS  CAS  Google Scholar 

  24. Lamb, J. R., Eckels, D. D., Lack, P., Woody, J. U. & Green, N. Nature 300, 66–69 (1982).

    Article  ADS  CAS  Google Scholar 

  25. Farrar, W. L. & Ruscetti, F. W. J. Immun. 136, 1266–1273 (1985).

    Google Scholar 

  26. Imboden, J. B. & Stobo, J. O. J. exp. Med. 161, 446–456 (1985).

    Article  CAS  Google Scholar 

  27. Oettgen, H. C., Pettey, C. L., Maloy, L. & Terhorst, C. Nature 320, 272–275 (1986).

    Article  ADS  CAS  Google Scholar 

  28. Reinherz, E. L. et al. Cell 30, 735–743 (1982).

    Article  CAS  Google Scholar 

  29. Zanders, E., Lamb, J., Feldman, M., Green, N. & Beverley, P. Nature 313, 318–320 (1983).

    Google Scholar 

  30. Delia, D. et al. Int. J. Cancer 29, 23–31 (1982).

    Article  CAS  Google Scholar 

  31. Ando, I., Hariri, G., Wallace, D. & Beverley, P. Euro. J. Immun. 15, 196–199 (1985).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Cell Surface Biochemistry, Imperial Cancer Research Fund, P.O. Box 123, Lincoln's Inn Fields, London, WC2A 3PX, UK

    D. Cantrell, A. A. Davies & M. J. Crumpton

  2. Charing Cross Sunley Research Centre, Lurgan Avenue, London, W6, UK

    M. Londei & M. Feldman

Authors
  1. D. Cantrell
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  2. A. A. Davies
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  3. M. Londei
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  4. M. Feldman
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  5. M. J. Crumpton
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Cantrell, D., Davies, A., Londei, M. et al. Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes. Nature 325, 540–542 (1987). https://doi.org/10.1038/325540a0

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