Abstract
Immunoglobulin gene promoters are active only in lymphoid cells and this tissue-specific activity requires an octamer sequence, ATTTGCAT (refs 1–9). Paradoxically, this same octamer motif seems to be a transcriptional control element in promoters which are active in all tissues10. Using an electrophoretic mobility shift assay to identify DNA binding proteins, we have now detected two species of nuclear proteins which bind specifically to this octamer. One previously characterized form11 (NF-Al) was found in all cell lines tested while the other form (NF-A2) was restricted to lymphoid cell lines. NF-A2 was found in cell lines representing all stages of B-cell differentiation and in half of the T-lymphoma cell lines tested. The identification of a lymphoid-specific octamer binding protein may account for the lymphoid-specific activity of immunoglobulin promoters.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Parslow, T. G., Blair, D. L., Murphy, W. J. & Granner, D. K. Proc. natn. Acad. Sci. U.S.A. 81, 2650–2654 (1984).
Bergman, Y., Rice, D., Grosschedl, R. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 81, 7041–7045 (1984).
Falkner, F. G. & Zachau, H. G. Nature 310, 71–74 (1984).
Grosschedl, R. & Baltimore, D. Cell 41, 885–897 (1985).
Mason, J. O., Williams, G. T. & Neuberger, M. S. Cell 41, 479–487 (1985).
Foster, J., Stafford, J. & Queen, C. Nature 315, 423–425 (1985).
Queen, C. & Stafford, J. Molec. cell. Biol. 4, 1042–1049 (1984).
Gopal, V. T., Shimada, T., Baur, A. W. & Nienhuis, A. W. Science 229, 1102–1104 (1985).
Picard, D. & Schaffner, W. EMBO J. 4, 2831–2838 (1985).
Sive, Hazel, L., Heintz, N. & Roeder, R. G. Molec. cell. Biol. (in the press).
Singh, H., Sen, R., Baltimore, D. & Sharp, P. A. Nature 319, 154–158 (1986).
Paige, C. J., Kincade, P. W. & Ralph, P. J. Immun. 121, 641–647 (1978).
Rosenberg, N., Siden, E. & Baltimore, D. in B Lymphocytes in the Immune Response (eds Cooper, M., Mosier, D., Scher, I. & Vitteta, E.) 379–386 (Eisevier, Amsterdam, 1979).
Perry, R. P. & Kelley, D. E. Cell 18, 1333–1339 (1979).
Alt, F. A., Rosenberg, N., Casanova, R. J., Thomas, E. & Baltimore, D. Nature 296, 325–331 (1982).
Lanier, L. L. J. Immun. 129, 1130–1137 (1982).
Nelson, K. J., Kelley, D. E. & Perry, R. P. Proc. natn. Acad. Sci. U.S.A. 82, 5305–5309 (1985).
Wall, R. et al. Proc. natn. Acad. Sci. U.S.A. 83, 295–298 (1986).
Lewis, S., Rosenberg, N., Alt, A. & Baltimore, D. Cell 30, 807–816 (1982).
Wasylyk, C. & Wasylyk, B. EMBO J. 5, 553–560 (1986).
Sen, R. & Baltimore, D. Cell (in the press).
Gerster, T., Picard, D. & Schaffner, W. Cell 45, 45–52 (1986).
Pierce, J. H. & Aaronson, S. A. J. exp. Med. 156, 873–887 (1982).
Braun, J. J. Immun. 130, 2113–2116 (1983).
Dignam, J. D., Lebowitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1489 (1983).
Maxam, A. & Gilbert, W. Meth. Enzym. 65, 449–525 (1980).
Dretzen, G., Bellard, M., Sassone-Corsi, P. & Chambon, P. Analyt. Biochem. 112, 295–298 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Staudt, L., Singh, H., Sen, R. et al. A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes. Nature 323, 640–643 (1986). https://doi.org/10.1038/323640a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/323640a0
- Springer Nature Limited
This article is cited by
-
Diversity among POU transcription factors in chromatin recognition and cell fate reprogramming
Cellular and Molecular Life Sciences (2018)
-
Non-conventional functions for NF-κB members: the dark side of NF-κB
Oncogene (2015)
-
Analysis of transcription factor OCT.1, OCT.2 and BOB.1 expression using tissue arrays in classical Hodgkin's lymphoma
Modern Pathology (2004)
-
Analysis of Octamer-Binding Transcription Factors Oct2 and Oct1 and their coactivator BOB.1/OBF.1 in Lymphomas
Modern Pathology (2002)
-
B-cell-specif ic coactivator OBF-1/OCA-B/Bob1 required for immune response and germinal centre formation
Nature (1996)