Skip to main content
SpringerLink
Log in

The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Classical phenomenological approaches to the study of the mechanism of T-cell-mediated cytotoxicity1–3 have now given way to a search for molecules involved in this function; this is attempted either by subcellular and biochemical fractionation of material from cytotoxic cells4,5, or through the characterization of molecules recognized by cytotoxicity-inhibiting monoclonal antibodies (see ref. 6 for a review). Molecules having a role in cytotoxi-city may also be identified by detecting the corresponding messenger RNA transcripts. Such an approach may include, as a first step, the search for transcripts as specific as possible to cytotoxic T cells; only secondarily can their actual relevance to cytotoxicity be investigated. We report here the preparation and systematic screening of a differential complementary DNA bank, in which we detected three distinct messenger RNA transcripts (CTLA-1, CTLA-2 and CTLA-3) present in various cytotoxic T cells but not (or less so) in a range of non-cytotoxic lymphoid cells. We describe the co-inducibility of these transcripts and of cytoxicity in thymocytes and hybridoma cells, the sequence of CTLA-1 cDNA, its protein homology with serine esterases and the localization of the corresponding gene to mouse chromosome 14.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Henney, C. S. Contemp. Topics Immunobiol. 7, 245–272 (1977).

    Article  CAS  Google Scholar 

  2. Golstein, P. & Smith, E. T. Contemp. Topics Immunobiol. 7, 273–300 (1977).

    Article  CAS  Google Scholar 

  3. Martz, E. Contemp. Topics Immunobiol. 7, 301–361 (1977).

    Article  CAS  Google Scholar 

  4. Henkart, P. A., Millard, P. J., Reynolds, C. W. & Henkart, M. P. J exp. Med. 160, 75–93 (1984).

    Article  CAS  Google Scholar 

  5. Podack, E. R. & Konigsberg, P. J. J. exp. Med. 160, 695–710 (1984).

    Article  CAS  Google Scholar 

  6. Golstein, P. et al. Immun. Rev. 68, 5–42 (1982).

    Article  CAS  Google Scholar 

  7. Timberlake, W. E. Devl Biol. 78, 497–510 (1980).

    Article  CAS  Google Scholar 

  8. Yanagi, Y. et al. Nature 308, 145–149 (1984).

    Article  ADS  CAS  Google Scholar 

  9. Hedrick, S. M., Cohen, D. I., Nielsen, E. A. & Davis, M. Nature 308, 149–153 (1984).

    Article  ADS  CAS  Google Scholar 

  10. Saito, H. et al. Nature 309, 757–762 (1984).

    Article  ADS  CAS  Google Scholar 

  11. Sims, J. E., Tunnacliffe, A., Smith, W. J. & Rabbitts, T. H. Nature 312, 541–545 (1984).

    Article  ADS  CAS  Google Scholar 

  12. Albert, F., Buferne, M., Boyer, C. & Schmitt-Verhulst, A.-M. Immunogenetics 16, 533–549 (1982).

    Article  CAS  Google Scholar 

  13. Hamano, T., Kim, W. M. & Asofsky, R. J. Immun. 129, 1403–1406 (1982).

    CAS  PubMed  Google Scholar 

  14. Conzelmann, A., Corthésy, P., Gianfriglia, M., Silva, A. & Nabholz, M. Nature 298, 170–172 (1982).

    Article  ADS  CAS  Google Scholar 

  15. Kranz, D. M. et al. Science 227, 941–945 (1985).

    Article  ADS  CAS  Google Scholar 

  16. Pasternack, M. S. & Eisen, H. N. Nature 314, 743–745 (1985).

    Article  ADS  CAS  Google Scholar 

  17. Lobe, C. G., Havele, C. & Bleackly, R. C. Proc. natn. Acad. Sci. U.S.A. 83, 1448–1452 (1986).

    Article  ADS  CAS  Google Scholar 

  18. Izant, J. G. & Weintraub, H. Cell 36, 1007–1015 (1984).

    Article  CAS  Google Scholar 

  19. Rubinstein, J. L. R., Nicolas, J.-F. & Jacob, F. C.r. hebd. Acad. Séanc. Acad. Sci., Paris 299, 271–274 (1984).

    Google Scholar 

  20. Auffray, C. & Rougeon, R. Eur. J. Biochem. 107, 303–314 (1980).

    Article  CAS  Google Scholar 

  21. Geck, P. & Nasz, I. Analyt. Biochem. 135, 264–268 (1983).

    Article  CAS  Google Scholar 

  22. Maniatis, T., Fritsch, E. F. & Sambrook, J. in Molecular Cloning. A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  23. Hanahan, D. J. molec. Biol. 166, 557–580 (1983).

    Article  CAS  Google Scholar 

  24. Pont, S. et al. Eur. J. Immun. 15, 1222–1228 (1985).

    Article  CAS  Google Scholar 

  25. Feinberg, A. & Vogelstein, B. Analyt. Biochem. 137, 266–267 (1984).

    Article  CAS  Google Scholar 

  26. Erard, F. et al. J. exp. Med. 160, 584–599 (1984).

    Article  CAS  Google Scholar 

  27. Kaiser, K. & Murray, N. E. in DNA Cloning Vol. 1 (ed. Glover, D. M.) (IRL Press, Oxford, 1985).

  28. Woodbury, G., Katunuma, N., Kobayashi, K., Titani, K. & Neurath, H. Biochemistry 17, 811–819 (1978).

    Article  CAS  Google Scholar 

  29. Gubler, U. & Hoffman, B. J. Gene 25, 263–269 (1983).

    Article  CAS  Google Scholar 

  30. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  31. Chuvpilov, S. A. & Kravchenko, V. V. FEBS Lett. 179, 34–36 (1984).

    Article  Google Scholar 

  32. Nesbitt, M. N. & Francke, U. Chromosoma 41, 145–158 (1973).

    Article  CAS  Google Scholar 

  33. Mattéi, M. G. et al. Hum. Genet. 69, 268–271 (1985).

    Article  Google Scholar 

  34. Camargo, M. & Cervenka, J. Am. J. hum. Genet. 34, 757–780 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Author notes

  1. Marie-Geneviève Mattei: INSERM U.242, CHU Timone, 13385 Marseille Cedex 5, France.

  2. Markus Nabholz: Swiss Institute for Experimental Cancer Research, Epalinges CH-1066, Switzerland.

Authors and Affiliations

  1. Centre d'lmmunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288 Marseille Cédex, 9, France

    Jean-François Brunet, Magali Dosseto, François Denizot, Marie-Geneviève Mattei, William R. Clark, Tariq M. Haqqi, Pierre Ferrier, Markus Nabholz, Anne-Marie Schmitt-Verhulst, Marie-Françoise Luciani & Pierre Golstein

  2. Molecular Biology Institute, University of California, Los Angeles, California, 90024, USA

    William R. Clark

Authors
  1. Jean-François Brunet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Magali Dosseto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. François Denizot
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marie-Geneviève Mattei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. William R. Clark
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tariq M. Haqqi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Pierre Ferrier
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Markus Nabholz
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Anne-Marie Schmitt-Verhulst
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Marie-Françoise Luciani
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Pierre Golstein
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brunet, JF., Dosseto, M., Denizot, F. et al. The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14. Nature 322, 268–271 (1986). https://doi.org/10.1038/322268a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/322268a0

  • Springer Nature Limited

This article is cited by

Search

Navigation