Abstract
Splicing of the Tetrahymena ribosomal intron was first studied by Cech et al.1,2, who subsequently demonstrated that the intron RNA catalyses its own excision from a primary transcript to yield mature ribosomal RNA3–5. This intron shares several short conserved sequences and a common secondary structure with several other introns 6–9, some of which have also been shown to self-splice10,11. Here I show that the conserved core of the Tetrahymena intron can act in trans to catalyse the sequence-specific cleavage and addition of guanosine to a separate RNA substrate.
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Szostak, J. Enzymatic activity of the conserved core of a group I self-splicing intron. Nature 322, 83–86 (1986). https://doi.org/10.1038/322083a0
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DOI: https://doi.org/10.1038/322083a0
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